Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607034645_uram CAACTCTAACTGTCTAATAAACTCAACGATAG(+ATATAT)GTACCCTAACCTAGATGAGGTGAGCCAGTTT NGG 10 8 17 8 24 0 100 5 10
    240607034643_kkja TGCTAGAGTTATTGTTACAATAATACAGGCCG(+ATATAT)GTACCTACTGTACAGAGTTAAAACTATATGG NGG 10 8 17 8 24 0 100 5 10
    240607034642_v9uv TAAAGCCATATAGTTTTAACTCTGTACAGTAG(+ATATAT)GTACCGGCCTGTATTATTGTAACAATAACTC NGG 10 8 17 8 24 0 100 5 10
    240607034640_i3bu CCCCTTAACACCATCTCCAGCCTCATCCTAGG(+ATATAT)GTACCAGCTCAAAACAATACCGGGAGAAGCA NGG 10 8 17 8 24 0 100 5 10
    240607034639_uesa CCGCCTGCTTCTCCCGGTATTGTTTTGAGCTG(+ATATAT)GTACCCTAGGATGAGGCTGGAGATGGTGTTA NGG 10 8 17 8 24 0 100 5 10
    240607034638_wcas AGCAAGCTCGGGAGCTCCCAGAATCTGCTTAG(+ATATAT)GATCCACTCCAGGGCTTGGAGAGGCGATGAA NGG 10 8 17 8 24 0 100 5 10
    240607034636_pvcj GTTTGTTCATCGCCTCTCCAAGCCCTGGAGTG(+ATATAT)GATCCTAAGCAGATTCTGGGAGCTCCCGAGC NGG 10 8 17 8 24 0 100 5 10
    240607034635_k9o5 AGACCTGGATCTCACTTGGATAAGAGTTCTGG(+ATATAT)GATCCTGCCATGATCCTTGAGAAAGAAAAAT NGG 10 8 17 8 24 0 100 5 10
    240607034633_imbk GCACGATTTTTCTTTCTCAAGGATCATGGCAG(+ATATAT)GATCCCAGAACTCTTATCCAAGTGAGATCCA NGG 10 8 17 8 24 0 100 5 10
    240607034631_k8hu ACAGGGCCTTACTGGCTCAGGGATTTGCCTGG(+ATATAT)GTACCTATGATTTCTCTTTCCCTACCCCATG NGG 10 8 17 8 24 0 100 5 10