Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607034630_ts28 ATCAACATGGGGTAGGGAAAGAGAAATCATAG(+ATATAT)GTACCCAGGCAAATCCCTGAGCCAGTAAGGC NGG 10 8 17 8 24 0 100 5 10
    240607034628_y13i AGGTTAGCAGTGGCAAGCTCACATAGAAGTGG(+ATATAT)GTACCACAGGGGTGAAGTAAGAGGTGCACCC NGG 10 8 17 8 24 0 100 5 10
    240607034627_h7oc TCTGTGGGTGCACCTCTTACTTCACCCCTGTG(+ATATAT)GTACCCACTTCTATGTGAGCTTGCCACTGCT NGG 10 8 17 8 24 0 100 5 10
    240607034625_ciio CCTTAAGCTGTACTACCCTTTTCTAGACATGG(+ATATAT)GATCCAGGTGTCCTGGTATCAGAGATCCTTG NGG 10 8 17 8 24 0 100 5 10
    240607034623_92vx GAGGGCAAGGATCTCTGATACCAGGACACCTG(+ATATAT)GATCCCATGTCTAGAAAAGGGTAGTACAGCT NGG 10 8 17 8 24 0 100 5 10
    240607034622_8l7p TAATCTCAGTGCTGTGGAGGCAGAGACAGGAG(+ATATAT)GATCCCTGAGCAGAAAAGCCCTACTGATGAG NGG 10 8 17 8 24 0 100 5 10
    240607034620_nhgl TGGAGCTCATCAGTAGGGCTTTTCTGCTCAGG(+ATATAT)GATCCTCCTGTCTCTGCCTCCACAGCACTGA NGG 10 8 17 8 24 0 100 5 10
    240607034618_4dez CTCAGGGAAGAGTGGTCACAGAGGGACAGAGG(+ATATAT)GATCCCACCCCCACCCCCACCCCCACCTGGA NGG 10 8 17 8 24 0 100 5 10
    240607034617_k2kw ATCGCTCCAGGTGGGGGTGGGGGTGGGGGTGG(+ATATAT)GATCCCTCTGTCCCTCTGTGACCACTCTTCC NGG 10 8 17 8 24 0 100 5 10
    240607034615_zsdk GGACAAGGTTTTCAGGTATATCTTTAAACAAG(+ATATAT)GATCCAAGACTCCGTGTCCTACAGGCTATTG NGG 10 8 17 8 24 0 100 5 10