Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607034601_ggw4 CAAGGCTTCAGTGTCGATAGGACAGCAATGTG(+ATATAT)GATCCCTCTCTCTGCTTGAAGCCCACTGGTT NGG 10 8 17 8 24 0 100 5 10
    240607034559_unhe CTCCTGGAAAGCCAGAGAGAGCTACATAGTTG(+ATATAT)GATCCAGTTTCAACCCCCCCTGTTCCCTACC NGG 10 8 17 8 24 0 100 5 10
    240607034558_0ykw CCCTTGGTAGGGAACAGGGGGGGTTGAAACTG(+ATATAT)GATCCAACTATGTAGCTCTCTCTGGCTTTCC NGG 10 8 17 8 24 0 100 5 10
    240607034557_epjk ACACCGCTAAGGTATTTTAAACCATGTTCTAG(+ATATAT)GATCCTTGAATTACCCAGTACAACATGTAAA NGG 10 8 17 8 24 0 100 5 10
    240607034556_wy9s AGCTATTTACATGTTGTACTGGGTAATTCAAG(+ATATAT)GATCCTAGAACATGGTTTAAAATACCTTAGC NGG 10 8 17 8 24 0 100 5 10
    240607034555_0kfi CGTGTGAGACCTGGTAAGAAAACTTCCACAAG(+ATATAT)GATCCTTCCTACAGTGGAATGTCAGGCCATC NGG 10 8 17 8 24 0 100 5 10
    240607034553_2m2w GCTGTGATGGCCTGACATTCCACTGTAGGAAG(+ATATAT)GATCCTTGTGGAAGTTTTCTTACCAGGTCTC NGG 10 8 17 8 24 0 100 5 10
    240607034552_c3gn GTAAGGTGGAGTTCGAAAGAGGGGCTAGTGTG(+ATATAT)GTACCCCAAAACAACACACAACCCTGCTCTG NGG 10 8 17 8 24 0 100 5 10
    240607034550_zdh6 ACAGCCAGAGCAGGGTTGTGTGTTGTTTTGGG(+ATATAT)GTACCACACTAGCCCCTCTTTCGAACTCCAC NGG 10 8 17 8 24 0 100 5 10
    240607034548_vlx2 CTATTCTCCTTCCAGCCAGCCAGAAATCCTCG(+ATATAT)GTACCTGCACCAGTGACCCAGCAGTCGCACA NGG 10 8 17 8 24 0 100 5 10