Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607034533_j5zd ACAGAAAATAGCATCACCAGAAGAGGGCATCG(+ATATAT)GATCCCATTACAGATGGTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240607034531_r3u8 GGATCTAGGGAATGAATAGTAGACCGTCAGTG(+ATATAT)GTACCATTACGTACAGTTGGTTACAGCACCG NGG 10 8 17 8 24 0 100 5 10
    240607034530_tovw TGGCACGGTGCTGTAACCAACTGTACGTAATG(+ATATAT)GTACCACTGACGGTCTACTATTCATTCCCTA NGG 10 8 17 8 24 0 100 5 10
    240607034529_gc20 CCAGGCAACAGAAACGCACATTAATGGTGACG(+ATATAT)GTACCGCTCACATGTGCTCACTACGAGTGTC NGG 10 8 17 8 24 0 100 5 10
    240607034528_w8zl TGTGAGACACTCGTAGTGAGCACATGTGAGCG(+ATATAT)GTACCGTCACCATTAATGTGCGTTTCTGTTG NGG 10 8 17 8 24 0 100 5 10
    240607034526_voc1 TCATGTATAAATGTAGACCATGCATGTGCCTG(+ATATAT)GTACCTGCAGAAGCCAGTAGAGTGCAGTGGA NGG 10 8 17 8 24 0 100 5 10
    240607034525_5cyo AAGGGTCCACTGCACTCTACTGGCTTCTGCAG(+ATATAT)GTACCAGGCACATGCATGGTCTACATTTATA NGG 10 8 17 8 24 0 100 5 10
    240607034524_ri8c GTCCAAGTGTAGGCAACCTTTCTTGGTTGATG(+ATATAT)GATCCAGTCTCTTGTGACAGCTGTCTGTGGG NGG 10 8 17 8 24 0 100 5 10
    240607034523_4v4j CAGTGCCCACAGACAGCTGTCACAAGAGACTG(+ATATAT)GATCCATCAACCAAGAAAGGTTGCCTACACT NGG 10 8 17 8 24 0 100 5 10
    240607034522_9gj1 GTGAAAGCACTTTGTCACTTACAGTATCTGAG(+ATATAT)GATCCCCTGCTACACTGATGGGAGGTGGGGA NGG 10 8 17 8 24 0 100 5 10