Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607034417_q5nf TCCCCAGCACTCACATGAAAGGCCTGGCTTGG(+ATATAT)GATCCCAGTGCTGGAGAGGCAGAGGCAGCGT NGG 10 8 17 8 24 0 100 5 10
    240607034415_khri TGCTCCACCCACACAGGTGCAGCAATATGGGG(+ATATAT)GTACCACCTCAAATGCTTTGTCATGCAGAGA NGG 10 8 17 8 24 0 100 5 10
    240607034414_aaxr AAGGATCTCTGCATGACAAAGCATTTGAGGTG(+ATATAT)GTACCCCCATATTGCTGCACCTGTGTGGGTG NGG 10 8 17 8 24 0 100 5 10
    240607034412_e8it TCAAACCACTGAGCTACTACACAATGTGGAGG(+ATATAT)GATCCATTTAAATCAATAAAACGACAGCCCA NGG 10 8 17 8 24 0 100 5 10
    240607034411_zu0v AACAATGGGCTGTCGTTTTATTGATTTAAATG(+ATATAT)GATCCCTCCACATTGTGTAGTAGCTCAGTGG NGG 10 8 17 8 24 0 100 5 10
    240607034409_lokd AGGCTGGATGTGCACAGCTAGTTGGGCGGATG(+ATATAT)GTACCAAGTCCTATGGAAACTGTTCTATTCC NGG 10 8 17 8 24 0 100 5 10
    240607034408_yj2w CCAAAGGAATAGAACAGTTTCCATAGGACTTG(+ATATAT)GTACCATCCGCCCAACTAGCTGTGCACATCC NGG 10 8 17 8 24 0 100 5 10
    240607034406_e499 GGGTGGGCTGACCGTAAACTGACCAGCGCTGG(+ATATAT)GATCCCACACTTGTTTTATACTCATCTCTAG NGG 10 8 17 8 24 0 100 5 10
    240607034405_8u1y GTAACCTAGAGATGAGTATAAAACAAGTGTGG(+ATATAT)GATCCCAGCGCTGGTCAGTTTACGGTCAGCC NGG 10 8 17 8 24 0 100 5 10
    240607034404_aqs5 TAATGTGTCATGTGTTATATTAATCTCTATTG(+ATATAT)GTACCTGACATTGTTTCTTTGAGCAGTATGA NGG 10 8 17 8 24 0 100 5 10