Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607033746_xbuz AATACTCTATAAACACTCTTTTCTACACTGTG(+ATATAT)GATCCTATACACAGTGTAGACAAACAGACTA NGG 10 8 17 8 24 0 100 5 10
    240607033745_lzt8 CTGGAAGCAAGCCCCCTCCTGGCATCTCTAGG(+ATATAT)GTACCTGGGCCCACATTCCTGTGGCCCACAA NGG 10 8 17 8 24 0 100 5 10
    240607033742_c5b0 CCCATTTGTGGGCCACAGGAATGTGGGCCCAG(+ATATAT)GTACCCTAGAGATGCCAGGAGGGGGCTTGCT NGG 10 8 17 8 24 0 100 5 10
    240607033740_p1t3 TTCCTGGAAGCCTGCCCCCAGCCCAGCCAGGG(+ATATAT)GATCCAGTCAGCAGCGTGGAGGGATTCAAGT NGG 10 8 17 8 24 0 100 5 10
    240607033738_hdb3 AAGGCACTTGAATCCCTCCACGCTGCTGACTG(+ATATAT)GATCCCCTGGCTGGGCTGGGGGCAGGCTTCC NGG 10 8 17 8 24 0 100 5 10
    240607033736_d9w3 GTGCCACCCAGGGTGCTGTTCCCTCTTGTGGG(+ATATAT)GATCCAGAGTAAAACTGGCTCGGCGCCAGGT NGG 10 8 17 8 24 0 100 5 10
    240607033735_jcsz ATCCCACCTGGCGCCGAGCCAGTTTTACTCTG(+ATATAT)GATCCCCACAAGAGGGAACAGCACCCTGGGT NGG 10 8 17 8 24 0 100 5 10
    240607033734_4k3b TCTTACCCACTCATGGAGTCATGGAGTTACAG(+ATATAT)GTACCTTCCTGAGCAGGGGATGGTAGCTCCT NGG 10 8 17 8 24 0 100 5 10
    240607033733_p4di TATTCAGGAGCTACCATCCCCTGCTCAGGAAG(+ATATAT)GTACCTGTAACTCCATGACTCCATGAGTGGG NGG 10 8 17 8 24 0 100 5 10
    240607033730_e0ui CAGATCTGCAATTCTCTGTTCTCTCGGCCTGG(+ATATAT)GATCCCAGCTCTCAGCAATCAAAATTGTTGC NGG 10 8 17 8 24 0 100 5 10