Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607033641_t17s ACAGGGAGCTAGGACCTTGTGCTTTTTCATGG(+ATATAT)GTACCAATAGAAGGTCAAGTAGGGGAAAAGG NGG 10 8 17 8 24 0 100 5 10
    240607033640_ypap CCTCTTCCCCAGTCAGTGGACAATCTTTCCAG(+ATATAT)GATCCCTGCTCATCCTTACAGATCATCTGGC NGG 10 8 17 8 24 0 100 5 10
    240607033638_4cli AGTCTGCCAGATGATCTGTAAGGATGAGCAGG(+ATATAT)GATCCTGGAAAGATTGTCCACTGACTGGGGA NGG 10 8 17 8 24 0 100 5 10
    240607033637_6lwo AAAACAAAACAACAACAACAAAAACCCAGCAG(+ATATAT)GTACCATGCTAAGACAACACCACATGCTTAA NGG 10 8 17 8 24 0 100 5 10
    240607033636_rmb8 CCCCTTTAAGCATGTGGTGTTGTCTTAGCATG(+ATATAT)GTACCTGCTGGGTTTTTGTTGTTGTTGTTTT NGG 10 8 17 8 24 0 100 5 10
    240607033634_kncd ATAATATCAGTTTTATTAAATATTCGAGAACG(+ATATAT)GATCCAGAGGTCGGATTTATACAGGAGGATA NGG 10 8 17 8 24 0 100 5 10
    240607033632_pi4r CGTGTTATCCTCCTGTATAAATCCGACCTCTG(+ATATAT)GATCCGTTCTCGAATATTTAATAAAACTGAT NGG 10 8 17 8 24 0 100 5 10
    240607033631_xmqh AAGGCCAGGGGTTTAGAGACAGTCTTCCTCTG(+ATATAT)GTACCCCATTCGAACCCACCTCTACAGCTGC NGG 10 8 17 8 24 0 100 5 10
    240607033629_4ap9 CATCCGCAGCTGTAGAGGTGGGTTCGAATGGG(+ATATAT)GTACCAGAGGAAGACTGTCTCTAAACCCCTG NGG 10 8 17 8 24 0 100 5 10
    240607033628_oc2x TTTGTGCCTTCTGTGAGGGATAGGGGACAAGG(+ATATAT)GATCCCAGGTGTCCCCATTTCCCGCCCCCTC NGG 10 8 17 8 24 0 100 5 10