Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607033626_bx1y AAGGTGAGGGGGCGGGAAATGGGGACACCTGG(+ATATAT)GATCCCTTGTCCCCTATCCCTCACAGAAGGC NGG 10 8 17 8 24 0 100 5 10
    240607033624_drqa TGTGTCAGAGGTAGAGATGGCACCTCTGGAGG(+ATATAT)GTACCAGAGCTAGGTGGATACATGGACCCAG NGG 10 8 17 8 24 0 100 5 10
    240607033623_tmcy TGCCCCTGGGTCCATGTATCCACCTAGCTCTG(+ATATAT)GTACCCTCCAGAGGTGCCATCTCTACCTCTG NGG 10 8 17 8 24 0 100 5 10
    240607033621_tc2m CTCTTCAAAATCCAAAACCTGTGGCCTTACTG(+ATATAT)GTACCAAATTCTCCTTGTAAACATACCTGGC NGG 10 8 17 8 24 0 100 5 10
    240607033620_cdyu CTGTAGCCAGGTATGTTTACAAGGAGAATTTG(+ATATAT)GTACCAGTAAGGCCACAGGTTTTGGATTTTG NGG 10 8 17 8 24 0 100 5 10
    240607033618_i4qd GCTGAGGCAGGCACACTATCCTCACTCCCTCG(+ATATAT)GTACCTGATCTCTAAATCACTTCTCAACTGA NGG 10 8 17 8 24 0 100 5 10
    240607033617_dfvk TGGGATCAGTTGAGAAGTGATTTAGAGATCAG(+ATATAT)GTACCGAGGGAGTGAGGATAGTGTGCCTGCC NGG 10 8 17 8 24 0 100 5 10
    240607033614_82bh CTGGACTACATATAAATTCAGATCAGCCAAGG(+ATATAT)GTACCTAGAAAGACCTATTTCAAAACAAAAA NGG 10 8 17 8 24 0 100 5 10
    240607033613_igw5 CTTGTTTTTTGTTTTGAAATAGGTCTTTCTAG(+ATATAT)GTACCCTTGGCTGATCTGAATTTATATGTAG NGG 10 8 17 8 24 0 100 5 10
    240607033612_7v5j CTGAGCCTGTGGTCAGCTGACCACAGAGGTGG(+ATATAT)GTACCTGAGTCCCCATGGCCCCCTGGAAGGT NGG 10 8 17 8 24 0 100 5 10