Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607033522_dwef ATATGAAAGAAAGAATTCAGGTACAGACCAGG(+ATATAT)GATCCCCAGTTTGCAGATCAGGTCAGGAAAC NGG 10 8 17 8 24 0 100 5 10
    240607033521_g40c CCCTCACCCCAGAGTAAGCTGCACTGTTCTTG(+ATATAT)GTACCAGAGATCCGCTAGTGCACAAAGACTC NGG 10 8 17 8 24 0 100 5 10
    240607033520_7ryy TTACCGAGTCTTTGTGCACTAGCGGATCTCTG(+ATATAT)GTACCAAGAACAGTGCAGCTTACTCTGGGGT NGG 10 8 17 8 24 0 100 5 10
    240607033519_gcud TGCAGCCGCAGCACCCGAGTCTGGAGAGAGAG(+ATATAT)GATCCCTTGAGCACAGGGATTGGAGGCCAGC NGG 10 8 17 8 24 0 100 5 10
    240607033517_55dh CCCAGGCTGGCCTCCAATCCCTGTGCTCAAGG(+ATATAT)GATCCTCTCTCTCCAGACTCGGGTGCTGCGG NGG 10 8 17 8 24 0 100 5 10
    240607033516_f1f5 TGTGACTGAGCTAGCCAGCCAGTAAGTTCAAG(+ATATAT)GATCCTCCTGGCCTCGCGCCCCAACACTGTG NGG 10 8 17 8 24 0 100 5 10
    240607033514_7r1w GGGACCACAGTGTTGGGGCGCGAGGCCAGGAG(+ATATAT)GATCCTTGAACTTACTGGCTGGCTAGCTCAG NGG 10 8 17 8 24 0 100 5 10
    240607033513_7igz ATCTTCATTCCTGAGCCACCTTGCCTCCTCTG(+ATATAT)GATCCCTTTTCTAAGAAAACACACTTGGTCT NGG 10 8 17 8 24 0 100 5 10
    240607033511_br8w AGTGCAGACCAAGTGTGTTTTCTTAGAAAAGG(+ATATAT)GATCCAGAGGAGGCAAGGTGGCTCAGGAATG NGG 10 8 17 8 24 0 100 5 10
    240607033510_t7s5 AAAATATATCGGCAGTCACTTCTTTTATAAAG(+ATATAT)GTACCATGGGCTACATTACACAATAGTCTCA NGG 10 8 17 8 24 0 100 5 10