Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607033422_627k GGGCTGTCTCTGACTCGTTCACCTGCTCTGGG(+ATATAT)GATCCTTTCCTCCTACGGCATTGTCCAGCCC NGG 10 8 17 8 24 0 100 5 10
    240607033420_8fuw GCTGTCTTCAGACACACCAGAAGAGGGCATCG(+ATATAT)GATCCCACTAGATGGTTGTGAGCCATCATGT NGG 10 8 17 8 24 0 100 5 10
    240607033419_4jtn CAACCACATGATGGCTCACAACCATCTAGTGG(+ATATAT)GATCCGATGCCCTCTTCTGGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240607033418_5pnd GACCACCACACCCCGGCGGAAAAGCTGTCTTG(+ATATAT)GATCCCTTTCTAGGAGTTACAGTGGCTGTCT NGG 10 8 17 8 24 0 100 5 10
    240607033416_4jkx CCTGGAGACAGCCACTGTAACTCCTAGAAAGG(+ATATAT)GATCCAAGACAGCTTTTCCGCCGGGGTGTGG NGG 10 8 17 8 24 0 100 5 10
    240607033414_fzkk GCATGGTGTGCTTTCTGACTGGGGTAGTGTGG(+ATATAT)GATCCCTCTCTGAGGACCCCGATGTTGGTAC NGG 10 8 17 8 24 0 100 5 10
    240607033412_uy62 CCCTAGTACCAACATCGGGGTCCTCAGAGAGG(+ATATAT)GATCCCACACTACCCCAGTCAGAAAGCACAC NGG 10 8 17 8 24 0 100 5 10
    240607033411_7ozd TGGAAACTTGGAAGAAGAGTGGTACTGTGATG(+ATATAT)GTACCTGATGGAGTGGTCCAAATTCCCAGTT NGG 10 8 17 8 24 0 100 5 10
    240607033410_iny7 GAAACAACTGGGAATTTGGACCACTCCATCAG(+ATATAT)GTACCATCACAGTACCACTCTTCTTCCAAGT NGG 10 8 17 8 24 0 100 5 10
    240607033408_zvhr TCTGGAGAAACTCACGACAGAGGTGGTGCTTG(+ATATAT)GTACCTAGTTCCCAGGGCCAGGTAAACTTGT NGG 10 8 17 8 24 0 100 5 10