Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607033318_gyo3 GAGGATGGGCTGACCGGAGAAAGCTTAGAGTG(+ATATAT)GTACCCAAGCTTGAAATCACAGGTCTCACCT NGG 10 8 17 8 24 0 100 5 10
    240607033317_3zky CCAAAATGGAATTGCTGTTTCCCAAACTTTGG(+ATATAT)GATCCTTGAAGGGAGAGCTGAACATTAATAT NGG 10 8 17 8 24 0 100 5 10
    240607033316_96zb TGCTCATATTAATGTTCAGCTCTCCCTTCAAG(+ATATAT)GATCCCAAAGTTTGGGAAACAGCAATTCCAT NGG 10 8 17 8 24 0 100 5 10
    240607033314_9rx7 AATCAAGCTCTCCCAATAAACGTGTGCAGAAG(+ATATAT)GATCCTGTTGCAGCGTCGTTCTTGCTGGTCA NGG 10 8 17 8 24 0 100 5 10
    240607033313_1mo4 AGACTTGACCAGCAAGAACGACGCTGCAACAG(+ATATAT)GATCCTTCTGCACACGTTTATTGGGAGAGCT NGG 10 8 17 8 24 0 100 5 10
    240607033312_a9lw GGCCTTTGCTGAGGCATTCCTTTCCCTCTGAG(+ATATAT)GTACCAGTCACAGGACGGGATAATATAGAAT NGG 10 8 17 8 24 0 100 5 10
    240607033311_8pe8 ACTCTATTCTATATTATCCCGTCCTGTGACTG(+ATATAT)GTACCTCAGAGGGAAAGGAATGCCTCAGCAA NGG 10 8 17 8 24 0 100 5 10
    240607033309_qzy6 GAGCAAGAGTTTATAGAGCTGGGGGATGTACG(+ATATAT)GTACCTACCTCTGTATGTGACAGAGGACTCA NGG 10 8 17 8 24 0 100 5 10
    240607033308_dv38 GGATATGAGTCCTCTGTCACATACAGAGGTAG(+ATATAT)GTACCGTACATCCCCCAGCTCTATAAACTCT NGG 10 8 17 8 24 0 100 5 10
    240607033307_u89r CTCCTGGAAGAGAAAGGTCATAGAACACATAG(+ATATAT)GTACCCCACCCCTCTGGAAGGCAGAGGGTAA NGG 10 8 17 8 24 0 100 5 10