Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607033220_bt04 CCATGTGAGGAGTTACTGGTGTTTGTGTTCCG(+ATATAT)GTACCCAATAGCTGAGGTGCCCTGTGCTCTG NGG 10 8 17 8 24 0 100 5 10
    240607033219_nebb GAAAGAAAACCACATGTCTTGCCTCATCTGTG(+ATATAT)GATCCTGGCCTATAATGTGCACATGTAGACT NGG 10 8 17 8 24 0 100 5 10
    240607033217_4x1i CCCTGAGTCTACATGTGCACATTATAGGCCAG(+ATATAT)GATCCACAGATGAGGCAAGACATGTGGTTTT NGG 10 8 17 8 24 0 100 5 10
    240607033216_8zyg TGCAGGCACCAGGCACGAATGTGGAGAGCATG(+ATATAT)GATCCGTACAGGCACTCCCATATGCACAAAA NGG 10 8 17 8 24 0 100 5 10
    240607033215_2z6z TTATTTTTTGTGCATATGGGAGTGCCTGTACG(+ATATAT)GATCCATGCTCTCCACATTCGTGCCTGGTGC NGG 10 8 17 8 24 0 100 5 10
    240607033214_d1m8 GATTCCTGATTCCTTCTCAACATGTGTGTAAG(+ATATAT)GATCCTCGACCTGCAAAGTCCATCTCTTTCC NGG 10 8 17 8 24 0 100 5 10
    240607033212_png6 TTCATGGAAAGAGATGGACTTTGCAGGTCGAG(+ATATAT)GATCCTTACACACATGTTGAGAAGGAATCAG NGG 10 8 17 8 24 0 100 5 10
    240607033211_f49c GCCAACACCAAACGAATCAGTAGCAGCTGCTG(+ATATAT)GATCCAGCAGAAACCACAAGGCTCCACCGAA NGG 10 8 17 8 24 0 100 5 10
    240607033210_up8o GCAGATTCGGTGGAGCCTTGTGGTTTCTGCTG(+ATATAT)GATCCAGCAGCTGCTACTGATTCGTTTGGTG NGG 10 8 17 8 24 0 100 5 10
    240607033208_ugbj AGATCCTGACTTTGGCTCCAGCTATCAGTTGG(+ATATAT)GATCCTCAACGGACCACAATGAAGGTCCTCC NGG 10 8 17 8 24 0 100 5 10