Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607033207_27hd AAAGTGGAGGACCTTCATTGTGGTCCGTTGAG(+ATATAT)GATCCCAACTGATAGCTGGAGCCAAAGTCAG NGG 10 8 17 8 24 0 100 5 10
    240607033206_9kjs TGAAGTGTTGTGGAAGCCAAAAGAGAGTTTTG(+ATATAT)GATCCCCTGGAGTTCCTGGGGAGGAGAGCCA NGG 10 8 17 8 24 0 100 5 10
    240607033204_5z5t GAGTTTGGCTCTCCTCCCCAGGAACTCCAGGG(+ATATAT)GATCCAAAACTCTCTTTTGGCTTCCACAACA NGG 10 8 17 8 24 0 100 5 10
    240607033202_agza CATGTGCCTTCTGAGTCTAGAAGAGGGTATTG(+ATATAT)GATCCCATGGAGCTGAAATTATAGGCAGTCG NGG 10 8 17 8 24 0 100 5 10
    240607033200_wqx9 AGTCACGACTGCCTATAATTTCAGCTCCATGG(+ATATAT)GATCCAATACCCTCTTCTAGACTCAGAAGGC NGG 10 8 17 8 24 0 100 5 10
    240607033158_o5zd TCCAAAGAAGCTAGAAGTTTCCATTTCACAGG(+ATATAT)GATCCTGCCCAGCTCCTCGGGGTGGCTACCT NGG 10 8 17 8 24 0 100 5 10
    240607033157_0wi2 TTGTGAGGTAGCCACCCCGAGGAGCTGGGCAG(+ATATAT)GATCCCTGTGAAATGGAAACTTCTAGCTTCT NGG 10 8 17 8 24 0 100 5 10
    240607033156_l718 CCGTCCATCAGTTCCATCTCTGTCTTCTATAG(+ATATAT)GATCCCTTCTTAACAGTTTTTAGCATATTTT NGG 10 8 17 8 24 0 100 5 10
    240607033154_erwf CACTAAAAATATGCTAAAAACTGTTAAGAAGG(+ATATAT)GATCCTATAGAAGACAGAGATGGAACTGATG NGG 10 8 17 8 24 0 100 5 10
    240607033153_mlbm TATTTTAATATGTTATACATCAGTGTATGTAG(+ATATAT)GATCCAGAGCTTTCCTTCTGAACCTCACTTC NGG 10 8 17 8 24 0 100 5 10