Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607033104_d80x CATTTTCCTTTAGGCTCAAGAATTTCCTTTAG(+ATATAT)GATCCACTTGATAACTCAACCAGATCTCCCT NGG 10 8 17 8 24 0 100 5 10
    240607033102_taed AAGGCTCTAAAGTGAGTAGGTCATTCCCTAGG(+ATATAT)GATCCTACAGATCACCCCCTGTTAAATCTAC NGG 10 8 17 8 24 0 100 5 10
    240607033100_95fb TATAGGTAGATTTAACAGGGGGTGATCTGTAG(+ATATAT)GATCCCTAGGGAATGACCTACTCACTTTAGA NGG 10 8 17 8 24 0 100 5 10
    240607033059_shdi CCAGAGTGCCAAGCTCTCTGTTCCTCTCCAAG(+ATATAT)GATCCCCTGGCCCTCACTGTGACGGACGGAC NGG 10 8 17 8 24 0 100 5 10
    240607033056_8xyp ACTCCGTCCGTCCGTCACAGTGAGGGCCAGGG(+ATATAT)GATCCTTGGAGAGGAACAGAGAGCTTGGCAC NGG 10 8 17 8 24 0 100 5 10
    240607033055_572j CGTGCTCTTAGGTCGTTTTATTTTACAATCTG(+ATATAT)GATCCGAGGTGGCAGATTTAAGACAGGTTCT NGG 10 8 17 8 24 0 100 5 10
    240607033053_kt28 TCTACAGAACCTGTCTTAAATCTGCCACCTCG(+ATATAT)GATCCAGATTGTAAAATAAAACGACCTAAGA NGG 10 8 17 8 24 0 100 5 10
    240607033052_lmp0 ATGCAACAGCCTGCAAAGGCCATAGGGTGTTG(+ATATAT)GATCCCTGGAACTGGAGTTACAGGAGGTTGA NGG 10 8 17 8 24 0 100 5 10
    240607033049_6uei TTTCCTCAACCTCCTGTAACTCCAGTTCCAGG(+ATATAT)GATCCAACACCCTATGGCCTTTGCAGGCTGT NGG 10 8 17 8 24 0 100 5 10
    240607033048_u7c1 ACAAAAACCCTCTATTAAACACAACAGAAAAG(+ATATAT)GATCCACAGTGCATCCAGACAGTGGAGTTGG NGG 10 8 17 8 24 0 100 5 10