Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607033005_se7w GTGGGGGTCGGGGTGTGTGTGTGAATGACACG(+ATATAT)GATCCCTCATCCTGATGCAGGAAGATGGTGG NGG 10 8 17 8 24 0 100 5 10
    240607033003_kk9c CTTCCCGAGCTCCTTGTGAGTTTGGGGGCTAG(+ATATAT)GATCCCTTCCTGTGATCAAAGGTGGGACGAT NGG 10 8 17 8 24 0 100 5 10
    240607033002_2ggu AAGGGATCGTCCCACCTTTGATCACAGGAAGG(+ATATAT)GATCCTAGCCCCCAAACTCACAAGGAGCTCG NGG 10 8 17 8 24 0 100 5 10
    240607033000_y1kq GGGCCAGCTGACGAGTTCCCCATCATCCTGAG(+ATATAT)GATCCAACCCCACCTCAAGTTTCTCCTGGAG NGG 10 8 17 8 24 0 100 5 10
    240607032958_agcm GGAGGCTCCAGGAGAAACTTGAGGTGGGGTTG(+ATATAT)GATCCTCAGGATGATGGGGAACTCGTCAGCT NGG 10 8 17 8 24 0 100 5 10
    240607032957_vp20 GGGCCTGGGCCAAAACCAGCAAGGTATAGTGG(+ATATAT)GATCCAGGAGGGACTGAGGGAGCCAGGGGCC NGG 10 8 17 8 24 0 100 5 10
    240607032955_kz3x CCTTAGGCCCCTGGCTCCCTCAGTCCCTCCTG(+ATATAT)GATCCCACTATACCTTGCTGGTTTTGGCCCA NGG 10 8 17 8 24 0 100 5 10
    240607032952_9rtt CACGTGTCCTAATGATGAAGTCAGAGCCCCGG(+ATATAT)GATCCCAGCACACACCGTGACACAGCCCAGC NGG 10 8 17 8 24 0 100 5 10
    240607032950_fy44 CAGTGGCTGGGCTGTGTCACGGTGTGTGCTGG(+ATATAT)GATCCCGGGGCTCTGACTTCATCATTAGGAC NGG 10 8 17 8 24 0 100 5 10
    240607032949_973u CCGTCAATGCCGCCTCAGGATGCAGGTGTGTG(+ATATAT)GTACCATGGCCTTGGGCAGCCCGTTGGCCTG NGG 10 8 17 8 24 0 100 5 10