Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607032932_g18m AGGCTGGGACTCAGACACACATGTCAAGGGTG(+ATATAT)GTACCTGGAATCAGCAATACCTGGTGTGTGG NGG 10 8 17 8 24 0 100 5 10
    240607032930_ly6m CAGCTTTGTCAACAGTTCTGTATCCATACCTG(+ATATAT)GTACCCTCGGCCCCGCTTCCGAGTCGCTTTT NGG 10 8 17 8 24 0 100 5 10
    240607032928_g34x AAGTGAAAAGCGACTCGGAAGCGGGGCCGAGG(+ATATAT)GTACCAGGTATGGATACAGAACTGTTGACAA NGG 10 8 17 8 24 0 100 5 10
    240607032927_rhy9 AGAAGACACCCAGGGTGACGTCTCATCCTCAG(+ATATAT)GATCCCCCACGTCTGCTGCCATCACAGCCGC NGG 10 8 17 8 24 0 100 5 10
    240607032924_vz0j GGGATGCGGCTGTGATGGCAGCAGACGTGGGG(+ATATAT)GATCCTGAGGATGAGACGTCACCCTGGGTGT NGG 10 8 17 8 24 0 100 5 10
    240607032923_f6tc ACAGGGGTAAAGGTTAGACAGTGGCAACTTAG(+ATATAT)GATCCTGTTAGGGTCTGGCCCATGTTTTAAC NGG 10 8 17 8 24 0 100 5 10
    240607032922_ydaq TGGGAGTTAAAACATGGGCCAGACCCTAACAG(+ATATAT)GATCCTAAGTTGCCACTGTCTAACCTTTACC NGG 10 8 17 8 24 0 100 5 10
    240607032921_efz6 TAACTCATGCCCAGACACTGATGGAGGGGCAG(+ATATAT)GTACCCCCAAAACACCAAGCCCTAAGAGGCC NGG 10 8 17 8 24 0 100 5 10
    240607032918_ct3q GGTAGGGCCTCTTAGGGCTTGGTGTTTTGGGG(+ATATAT)GTACCTGCCCCTCCATCAGTGTCTGGGCATG NGG 10 8 17 8 24 0 100 5 10
    240607032917_zq37 TCTTGTGTGCCATCCTTCAGACTGGTTAAGAG(+ATATAT)GATCCTCTGCCCACCTGTGTCCACAGGTGGT NGG 10 8 17 8 24 0 100 5 10