Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607032844_rcac ATCACTACGGTAACCACAGAGACCCGCAGGTG(+ATATAT)GATCCATCCCTTCACCTCTGCTGCTCCTGGC NGG 10 8 17 8 24 0 100 5 10
    240607032843_06et CTCTGCATCCATCTTGATTAAAGATGGGTGTG(+ATATAT)GATCCTCTCCCACATCCTAGGGTGTGGGGCA NGG 10 8 17 8 24 0 100 5 10
    240607032841_ipp8 ATGGCTGCCCCACACCCTAGGATGTGGGAGAG(+ATATAT)GATCCACACCCATCTTTAATCAAGATGGATG NGG 10 8 17 8 24 0 100 5 10
    240607032840_2c8t CTCCCTTCAGCACGGTAGGTCTTTGCATCTAG(+ATATAT)GATCCCCGCCCCAGACCGCCTGTGAGCAGAG NGG 10 8 17 8 24 0 100 5 10
    240607032838_dy6v CTTTGCTCTGCTCACAGGCGGTCTGGGGCGGG(+ATATAT)GATCCTAGATGCAAAGACCTACCGTGCTGAA NGG 10 8 17 8 24 0 100 5 10
    240607032837_r6dl TGGTAGCCTGTGGTCAGGCAGCCCTTGTAATG(+ATATAT)GATCCCTACCTTCTTGTAAAGTCTCTAAAAA NGG 10 8 17 8 24 0 100 5 10
    240607032835_0jnu ACACATTTTTAGAGACTTTACAAGAAGGTAGG(+ATATAT)GATCCATTACAAGGGCTGCCTGACCACAGGC NGG 10 8 17 8 24 0 100 5 10
    240607032833_98lk AATACCCTCTTTCTGACCTCCACAGGCACAGG(+ATATAT)GTACCCATGTGGTATACATATATACACAAGG NGG 10 8 17 8 24 0 100 5 10
    240607032832_ts0q TTTTGCCTTGTGTATATATGTATACCACATGG(+ATATAT)GTACCCTGTGCCTGTGGAGGTCAGAAAGAGG NGG 10 8 17 8 24 0 100 5 10
    240607032829_jypi AAGGTGGCTGGCTGGGGAATCCATGGGGGGGG(+ATATAT)GATCCTGATTATAGATACTTCTGTTAGGGAT NGG 10 8 17 8 24 0 100 5 10