Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607032828_h6j6 GCTTTATCCCTAACAGAAGTATCTATAATCAG(+ATATAT)GATCCCCCCCCCATGGATTCCCCAGCCAGCC NGG 10 8 17 8 24 0 100 5 10
    240607032827_q3fc CTGTAGATAAAACAAAATTATACCGAGTAAAG(+ATATAT)GTACCAGGATATCTGGTCCCCAAGGCTGGAA NGG 10 8 17 8 24 0 100 5 10
    240607032825_lp1q CCTGGTTCCAGCCTTGGGGACCAGATATCCTG(+ATATAT)GTACCTTTACTCGGTATAATTTTGTTTTATC NGG 10 8 17 8 24 0 100 5 10
    240607032823_ko25 GGCTGTTCTGCATGGGAGGAAGTGGGTTAGGG(+ATATAT)GATCCAGGCTCTTGGCCAGACATACAGAGCT NGG 10 8 17 8 24 0 100 5 10
    240607032822_s17g ATGTCAGCTCTGTATGTCTGGCCAAGAGCCTG(+ATATAT)GATCCCCTAACCCACTTCCTCCCATGCAGAA NGG 10 8 17 8 24 0 100 5 10
    240607032820_i84b AATAAATAAATCTTTAGGAAAAAATCTGCAAG(+ATATAT)GTACCAGATGGAGGATTCGGGGTCAAGGTGA NGG 10 8 17 8 24 0 100 5 10
    240607032819_dr8u CCACATCACCTTGACCCCGAATCCTCCATCTG(+ATATAT)GTACCTTGCAGATTTTTTCCTAAAGATTTAT NGG 10 8 17 8 24 0 100 5 10
    240607032818_a21y GCTATGCAGAGTCCCATGGTCTCTGCATGTTG(+ATATAT)GTACCTCACCAAGTCCCACTGTGCTCTTGTG NGG 10 8 17 8 24 0 100 5 10
    240607032817_w7jv TCTACCACAAGAGCACAGTGGGACTTGGTGAG(+ATATAT)GTACCAACATGCAGAGACCATGGGACTCTGC NGG 10 8 17 8 24 0 100 5 10
    240607032815_i5ls CCACCATTGCTTCTGTAACTGCTGGGCGCTGG(+ATATAT)GATCCTGCCCCCAGGAAGTTAAGAGTGGGCA NGG 10 8 17 8 24 0 100 5 10