Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607032730_4f82 CTCATTTGCTTGCAGCCTTCCCCCTATTTCTG(+ATATAT)GATCCACCTGTGCTGTCCCCCCTCCCACTCC NGG 10 8 17 8 24 0 100 5 10
    240607032729_uuby TACTACAGACTCACACAGCCTTGTGTTAGGTG(+ATATAT)GTACCAATCTGTGTTTATTGAAAACCTACGT NGG 10 8 17 8 24 0 100 5 10
    240607032728_59hm GCTGGACGTAGGTTTTCAATAAACACAGATTG(+ATATAT)GTACCACCTAACACAAGGCTGTGTGAGTCTG NGG 10 8 17 8 24 0 100 5 10
    240607032726_enyk GGCAGTGGCCTGCAGCCTGCCCTCACCAAGGG(+ATATAT)GATCCAGGCCAGAAGTTCCTTCAGCCAGCCT NGG 10 8 17 8 24 0 100 5 10
    240607032724_s4py GGAGGAGGCTGGCTGAAGGAACTTCTGGCCTG(+ATATAT)GATCCCCTTGGTGAGGGCAGGCTGCAGGCCA NGG 10 8 17 8 24 0 100 5 10
    240607032723_oyan ACAACATACCAAAGGAATCTGCAATCCTGCAG(+ATATAT)GATCCAAATAACCAATTTTATTAGAGGCTCT NGG 10 8 17 8 24 0 100 5 10
    240607032722_u776 GTCTCAGAGCCTCTAATAAAATTGGTTATTTG(+ATATAT)GATCCTGCAGGATTGCAGATTCCTTTGGTAT NGG 10 8 17 8 24 0 100 5 10
    240607032720_mi57 CAGGAGACAGGAACACAAGCAGACAGGCCTGG(+ATATAT)GTACCGGAGACAGCAGTAAGGAACAGGCTCA NGG 10 8 17 8 24 0 100 5 10
    240607032716_z7st TCCTGTGAGCCTGTTCCTTACTGCTGTCTCCG(+ATATAT)GTACCCAGGCCTGTCTGCTTGTGTTCCTGTC NGG 10 8 17 8 24 0 100 5 10
    240607032715_nj0d AGGGCTGATGGCCCTGGGGTTCCTTGTCAAAG(+ATATAT)GATCCGCCTGTGTCTTTATTAACACAGTTCT NGG 10 8 17 8 24 0 100 5 10