Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607032658_5qjp CACCTAGGAAGCTTGAGCTTCCAGGCCCCAGG(+ATATAT)GTACCACTTCTGCATGGTGTTTAGGAAACTG NGG 10 8 17 8 24 0 100 5 10
    240607032656_p5lw TTCTGGAGCCTGGTTACTCCTTAGAATTCAAG(+ATATAT)GATCCAAGTGCAAGAGCTTCAGGCTTCAGCT NGG 10 8 17 8 24 0 100 5 10
    240607032655_dxke CTGACAGCTGAAGCCTGAAGCTCTTGCACTTG(+ATATAT)GATCCTTGAATTCTAAGGAGTAACCAGGCTC NGG 10 8 17 8 24 0 100 5 10
    240607032653_nbcy GGGAGGGTCTGCCCCCATTTTGACAGGCCAAG(+ATATAT)GATCCTGGGACCACTTGGGGTTGCAGTGTAT NGG 10 8 17 8 24 0 100 5 10
    240607032651_uz4b AGGTCATACACTGCAACCCCAAGTGGTCCCAG(+ATATAT)GATCCTTGGCCTGTCAAAATGGGGGCAGACC NGG 10 8 17 8 24 0 100 5 10
    240607032650_f8bf GGTGTCATTGCTTGGAGGCTAATTATGGGATG(+ATATAT)GATCCCTGGATATGGCAGTCTCTAGATGGAC NGG 10 8 17 8 24 0 100 5 10
    240607032647_zkv1 GGATGGTCCATCTAGAGACTGCCATATCCAGG(+ATATAT)GATCCATCCCATAATTAGCCTCCAAGCAATG NGG 10 8 17 8 24 0 100 5 10
    240607032645_4uta GGTGCACAGGCCTCGGAAGGCAGAGGCAGGGG(+ATATAT)GATCCCTACCGGTTTAGAATCTGCATGGGCT NGG 10 8 17 8 24 0 100 5 10
    240607032644_sd0r TATGCAGCCCATGCAGATTCTAAACCGGTAGG(+ATATAT)GATCCCCCTGCCTCTGCCTTCCGAGGCCTGT NGG 10 8 17 8 24 0 100 5 10
    240607032642_5llg TGTCTCGGTCCCGGCCGCTGCTCTCCAGCCAG(+ATATAT)GTACCCTCCTCACTCCCTGCTCGCGCAGTTC NGG 10 8 17 8 24 0 100 5 10