Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607032641_miz4 AGTGCGAACTGCGCGAGCAGGGAGTGAGGAGG(+ATATAT)GTACCTGGCTGGAGAGCAGCGGCCGGGACCG NGG 10 8 17 8 24 0 100 5 10
    240607032639_qnl6 GGCGCCGGGGGCGGTGCCGGCTCCAATCACGG(+ATATAT)GATCCGCCCAAGGGCCCCAACGCGGTAGGCG NGG 10 8 17 8 24 0 100 5 10
    240607032637_20ry GGAAGCGCCTACCGCGTTGGGGCCCTTGGGCG(+ATATAT)GATCCCGTGATTGGAGCCGGCACCGCCCCCG NGG 10 8 17 8 24 0 100 5 10
    240607032635_oz51 TCCTTCTTGCTCCGCTTCTATTCTGTCAAGGG(+ATATAT)GATCCCTGCAGTCTGAGGCTTCCCCCCCAAA NGG 10 8 17 8 24 0 100 5 10
    240607032634_2e5y CCAGGTTTGGGGGGGAAGCCTCAGACTGCAGG(+ATATAT)GATCCCCTTGACAGAATAGAAGCGGAGCAAG NGG 10 8 17 8 24 0 100 5 10
    240607032632_dyao TGGGTCCCAATGCATCTGCTGGGGGCTGAGAG(+ATATAT)GTACCTTCTGCCCATCACCAGCCTTGTTCTG NGG 10 8 17 8 24 0 100 5 10
    240607032631_m2i6 CAGCACAGAACAAGGCTGGTGATGGGCAGAAG(+ATATAT)GTACCTCTCAGCCCCCAGCAGATGCATTGGG NGG 10 8 17 8 24 0 100 5 10
    240607032630_7b2k TTCCTTTACTGAAGGAGGGCCAAGTCAGCATG(+ATATAT)GTACCACTGAGACAGATTTTTTTTTTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240607032629_reop TGTTTAAAAAAAAAAAAAAATCTGTCTCAGTG(+ATATAT)GTACCATGCTGACTTGGCCCTCCTTCAGTAA NGG 10 8 17 8 24 0 100 5 10
    240607032627_3cnu GCTGGAGTCTTAAAGTCAGAGGTTTCTCTTTG(+ATATAT)GATCCAATCAAGATTGAACAAATACTGAATG NGG 10 8 17 8 24 0 100 5 10