Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607032539_922x GGCACTTGAGAGGACACGTGCTTTCACACAGG(+ATATAT)GTACCAAAATATCTTCTGGGGACACCCCAAC NGG 10 8 17 8 24 0 100 5 10
    240607032538_yyvc AAGGTGTTGGGGTGTCCCCAGAAGATATTTTG(+ATATAT)GTACCCTGTGTGAAAGCACGTGTCCTCTCAA NGG 10 8 17 8 24 0 100 5 10
    240607032536_iylm ACCGAGGCTGCTGAGCTCTTCCAGCATGGAGG(+ATATAT)GTACCATGGCCCGTAGGAGCCTGGGGTTCCT NGG 10 8 17 8 24 0 100 5 10
    240607032534_xpq4 TTGCTAGGAACCCCAGGCTCCTACGGGCCATG(+ATATAT)GTACCCTCCATGCTGGAAGAGCTCAGCAGCC NGG 10 8 17 8 24 0 100 5 10
    240607032533_t0i3 CAGTTGGTGAGCAGTGGCCAGTGAGCAATGAG(+ATATAT)GATCCCATGGGTGGTCAAGTGGACCTGGGGG NGG 10 8 17 8 24 0 100 5 10
    240607032530_eufh AATGTCCCCCAGGTCCACTTGACCACCCATGG(+ATATAT)GATCCTCATTGCTCACTGGCCACTGCTCACC NGG 10 8 17 8 24 0 100 5 10
    240607032529_hp9d TCAGTCGGTCCCTCAACCCACGGGAAAATCAG(+ATATAT)GATCCCTGTACTCAAGTGGGCAAGGAGTCAG NGG 10 8 17 8 24 0 100 5 10
    240607032527_6e1t TTTTGCTGACTCCTTGCCCACTTGAGTACAGG(+ATATAT)GATCCTGATTTTCCCGTGGGTTGAGGGACCG NGG 10 8 17 8 24 0 100 5 10
    240607032526_e54d CATAAATGTGCCTGTCAGGTATTGGGGAAGGG(+ATATAT)GTACCCAGTGCCCTTCCTTCTGCCTACTAAA NGG 10 8 17 8 24 0 100 5 10
    240607032524_sjsz CTTACTTTAGTAGGCAGAAGGAAGGGCACTGG(+ATATAT)GTACCCCTTCCCCAATACCTGACAGGCACAT NGG 10 8 17 8 24 0 100 5 10