Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607032505_45th TAGTGGGTCATGGGCTCAGGTCCTTATCCCAG(+ATATAT)GATCCTCTAAGCCTTAGGTGTTGTGGCAAGA NGG 10 8 17 8 24 0 100 5 10
    240607032504_epni CAGACTCTTGCCACAACACCTAAGGCTTAGAG(+ATATAT)GATCCTGGGATAAGGACCTGAGCCCATGACC NGG 10 8 17 8 24 0 100 5 10
    240607032502_gpci AAAAGGAGTCATTCCACAATGTCTGTATGAGG(+ATATAT)GATCCAAACTCTGTCCTCTTTAATAAAGTCA NGG 10 8 17 8 24 0 100 5 10
    240607032501_modv TATTCTGACTTTATTAAAGAGGACAGAGTTTG(+ATATAT)GATCCCTCATACAGACATTGTGGAATGACTC NGG 10 8 17 8 24 0 100 5 10
    240607032459_w34m CAGTAGAACAGCACTGCTTGAGGCTCACATGG(+ATATAT)GTACCAAATCCCTCTATTTCCTGGCTTACTG NGG 10 8 17 8 24 0 100 5 10
    240607032458_xybr CAGGACAGTAAGCCAGGAAATAGAGGGATTTG(+ATATAT)GTACCCATGTGAGCCTCAAGCAGTGCTGTTC NGG 10 8 17 8 24 0 100 5 10
    240607032457_kso6 CACCACCCCAGAGGTCAGCCTTTGGTGCATGG(+ATATAT)GATCCAAGCCAGGACAAAGCACAAGCACAGA NGG 10 8 17 8 24 0 100 5 10
    240607032456_0onq TCCAGTCTGTGCTTGTGCTTTGTCCTGGCTTG(+ATATAT)GATCCCATGCACCAAAGGCTGACCTCTGGGG NGG 10 8 17 8 24 0 100 5 10
    240607032453_diub CATCACCCTGTGCTACCAGCATCTGGTCCCAG(+ATATAT)GATCCAGTCAGGCTAGGATTGTCAAAAGTTC NGG 10 8 17 8 24 0 100 5 10
    240607032452_ya92 CTTGTGAACTTTTGACAATCCTAGCCTGACTG(+ATATAT)GATCCTGGGACCAGATGCTGGTAGCACAGGG NGG 10 8 17 8 24 0 100 5 10