Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607032356_c0rm GTGCACATCAGTGATTTCTGTATATAAGGAGG(+ATATAT)GTACCAAGTACCCTCTACAAGGTTCCCCAGG NGG 10 8 17 8 24 0 100 5 10
    240607032355_md9m GGAGACCTGGGGAACCTTGTAGAGGGTACTTG(+ATATAT)GTACCCTCCTTATATACAGAAATCACTGATG NGG 10 8 17 8 24 0 100 5 10
    240607032353_el1s GTCTAGACTTAGTATCTGGGTGTGCTGTCCAG(+ATATAT)GATCCACAACTAAGCTTGTCTGCAGTTGAGA NGG 10 8 17 8 24 0 100 5 10
    240607032352_o56u CATGGTCTCAACTGCAGACAAGCTTAGTTGTG(+ATATAT)GATCCTGGACAGCACACCCAGATACTAAGTC NGG 10 8 17 8 24 0 100 5 10
    240607032351_rkty CATGGTGTCAACTGCAGACAAGCTTAGTTGTG(+ATATAT)GATCCCGGACAGCACACCCAGAGACTAAGTC NGG 10 8 17 8 24 0 100 5 10
    240607032349_2bj6 GTCTAGACTTAGTCTCTGGGTGTGCTGTCCGG(+ATATAT)GATCCACAACTAAGCTTGTCTGCAGTTGACA NGG 10 8 17 8 24 0 100 5 10
    240607032347_0ahj CCCCTGGTGCTAGATGCAAGGTCAAGAGGAGG(+ATATAT)GTACCTTCTGTCAGGTATGAGCTGCCTTCCA NGG 10 8 17 8 24 0 100 5 10
    240607032346_la6s GGATGTGGAAGGCAGCTCATACCTGACAGAAG(+ATATAT)GTACCCTCCTCTTGACCTTGCATCTAGCACC NGG 10 8 17 8 24 0 100 5 10
    240607032344_51id GTGTGTGTGTGTATCTGAGAATGTGGGTGTAG(+ATATAT)GTACCCTCCAAGGCCAGAAGAGGGCATCAGG NGG 10 8 17 8 24 0 100 5 10
    240607032343_l83n GGAGACCTGATGCCCTCTTCTGGCCTTGGAGG(+ATATAT)GTACCTACACCCACATTCTCAGATACACACA NGG 10 8 17 8 24 0 100 5 10