Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607032308_dqgc CGCATCTGTAGTACCAGAACTCAAGGCAGGAG(+ATATAT)GATCCTTGCTAAGTTTGAAGCCAGCCTAGGC NGG 10 8 17 8 24 0 100 5 10
    240607032307_jwsn AAGTAGCCTAGGCTGGCTTCAAACTTAGCAAG(+ATATAT)GATCCTCCTGCCTTGAGTTCTGGTACTACAG NGG 10 8 17 8 24 0 100 5 10
    240607032305_24yp GCCACTAGGCGAGTGGAGGAAGCTATAACCAG(+ATATAT)GATCCTGGGGGAGAGGGGCTGCCTACGGAGG NGG 10 8 17 8 24 0 100 5 10
    240607032302_4174 CCTTCCCTCCGTAGGCAGCCCCTCTCCCCCAG(+ATATAT)GATCCTGGTTATAGCTTCCTCCACTCGCCTA NGG 10 8 17 8 24 0 100 5 10
    240607032301_u3il GGGGAGGAGGGGGGGGGCTGAGAAGACTCCTG(+ATATAT)GTACCCAGTGAAAACACCTAGCCATGCATGA NGG 10 8 17 8 24 0 100 5 10
    240607032259_203r TGTTGTCATGCATGGCTAGGTGTTTTCACTGG(+ATATAT)GTACCAGGAGTCTTCTCAGCCCCCCCCCTCC NGG 10 8 17 8 24 0 100 5 10
    240607032258_7d6z TCGGATGGCAGAGCATTCTAGGAAGGGACGAG(+ATATAT)GATCCAAGTGTAGTCTGTCTTTTAAGGTCTT NGG 10 8 17 8 24 0 100 5 10
    240607032257_r1t8 TGCCCAAGACCTTAAAAGACAGACTACACTTG(+ATATAT)GATCCTCGTCCCTTCCTAGAATGCTCTGCCA NGG 10 8 17 8 24 0 100 5 10
    240607032256_8rtz AGGCCAGTCTGGTAGGCAGTTTTTCAATTGAG(+ATATAT)GATCCCTCTTCTCAGATTACTCTAGCTTGTA NGG 10 8 17 8 24 0 100 5 10
    240607032254_5qvb TTTGATACAAGCTAGAGTAATCTGAGAAGAGG(+ATATAT)GATCCTCAATTGAAAAACTGCCTACCAGACT NGG 10 8 17 8 24 0 100 5 10