Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607032240_e01v CCACCTGAGCCATATGCTGACCTCTTTGCCAG(+ATATAT)GATCCTCCATGCTGCTGTCCAGTAGGGCCCA NGG 10 8 17 8 24 0 100 5 10
    240607032238_nte7 GGACATGGGCCCTACTGGACAGCAGCATGGAG(+ATATAT)GATCCTGGCAAAGAGGTCAGCATATGGCTCA NGG 10 8 17 8 24 0 100 5 10
    240607032236_4dbc CTGCGCACCCCGCCGCCAAGCCCCAGCTCCAG(+ATATAT)GTACCTACCGCGGTGCGCCTCTCCCGCCGGG NGG 10 8 17 8 24 0 100 5 10
    240607032235_xcpa CGGGGCCCGGCGGGAGAGGCGCACCGCGGTAG(+ATATAT)GTACCTGGAGCTGGGGCTTGGCGGCGGGGTG NGG 10 8 17 8 24 0 100 5 10
    240607032232_pgf8 GATCCCTGATTCCGAGTCTGGGATTTCCCAGG(+ATATAT)GATCCTGGATGAGCTCAAGACACCCCGGGAA NGG 10 8 17 8 24 0 100 5 10
    240607032230_vob8 GTCTTTTCCCGGGGTGTCTTGAGCTCATCCAG(+ATATAT)GATCCCTGGGAAATCCCAGACTCGGAATCAG NGG 10 8 17 8 24 0 100 5 10
    240607032228_oe7k ACTAACAGCTAACTGTAATTCCACTTCCAGAG(+ATATAT)GATCCAACAGTATCTTCTGGCCTCTGACAAC NGG 10 8 17 8 24 0 100 5 10
    240607032227_q5mf GCAGTGTTGTCAGAGGCCAGAAGATACTGTTG(+ATATAT)GATCCTCTGGAAGTGGAATTACAGTTAGCTG NGG 10 8 17 8 24 0 100 5 10
    240607032226_koc7 CAGGGTGCCAGGGTCTGCTGAGGCTTTGGATG(+ATATAT)GATCCAGGCTACTTGTCTACGATGTGGCTGG NGG 10 8 17 8 24 0 100 5 10
    240607032224_ejp7 TTGTCCCAGCCACATCGTAGACAAGTAGCCTG(+ATATAT)GATCCATCCAAAGCCTCAGCAGACCCTGGCA NGG 10 8 17 8 24 0 100 5 10