Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607032325_gkqe CTAGCAGGGGGCTTGTACAGTGAGTGATGGGG(+ATATAT)GATCCCAGGAGAGTGCTGGTGATGGCTGGGT NGG 10 8 17 8 24 0 100 5 10
    240607032323_u7j3 CTCTGACCCAGCCATCACCAGCACTCTCCTGG(+ATATAT)GATCCCCCATCACTCACTGTACAAGCCCCCT NGG 10 8 17 8 24 0 100 5 10
    240607032322_co5h TACATCCTCATCACCAGTGCGGCTGGGTTCTG(+ATATAT)GTACCAGCTTCAGCACCCCACAGTACCAGGG NGG 10 8 17 8 24 0 100 5 10
    240607032321_c514 CTGAACCCTGGTACTGTGGGGTGCTGAAGCTG(+ATATAT)GTACCAGAACCCAGCCGCACTGGTGATGAGG NGG 10 8 17 8 24 0 100 5 10
    240607032319_ejn3 GCCGCGCCATATCTCGCGGCCAGTTCCTGCAG(+ATATAT)GTACCGGCTCGCCCTGCCAGTCGCAAGCCAG NGG 10 8 17 8 24 0 100 5 10
    240607032317_50od CGGGGCTGGCTTGCGACTGGCAGGGCGAGCCG(+ATATAT)GTACCTGCAGGAACTGGCCGCGAGATATGGC NGG 10 8 17 8 24 0 100 5 10
    240607032316_5rqn AGACCCACCGAGGGGGAAGAGTGTGTCAAGGG(+ATATAT)GTACCTAGGAGACCTGCTAAGCCGTCCCAGC NGG 10 8 17 8 24 0 100 5 10
    240607032314_bsqr CTGTTGCTGGGACGGCTTAGCAGGTCTCCTAG(+ATATAT)GTACCCCTTGACACACTCTTCCCCCTCGGTG NGG 10 8 17 8 24 0 100 5 10
    240607032312_zxi6 TTATAGTCCGTCATCCAGAGAAGTCAGGGCGG(+ATATAT)GATCCTGGAGGCAAGGACTGAAGCAGAGGCT NGG 10 8 17 8 24 0 100 5 10
    240607032310_afr5 TCCACAGCCTCTGCTTCAGTCCTTGCCTCCAG(+ATATAT)GATCCCGCCCTGACTTCTCTGGATGACGGAC NGG 10 8 17 8 24 0 100 5 10