Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607032713_g09f AACCCAGAACTGTGTTAATAAAGACACAGGCG(+ATATAT)GATCCTTTGACAAGGAACCCCAGGGCCATCA NGG 10 8 17 8 24 0 100 5 10
    240607032712_dndx GAGCCACCCTGAATCGTCTCCAGAGTGTCATG(+ATATAT)GATCCAGGCTGGCTTCTCACTTGCTGTATAG NGG 10 8 17 8 24 0 100 5 10
    240607032710_z0i3 CTATGCTATACAGCAAGTGAGAAGCCAGCCTG(+ATATAT)GATCCATGACACTCTGGAGACGATTCAGGGT NGG 10 8 17 8 24 0 100 5 10
    240607032709_ybcy TTTGTTTGCATGTATGTATGTGTGCATGCTTG(+ATATAT)GTACCTAGAGTTAAGGGCAGCAGACACCCCT NGG 10 8 17 8 24 0 100 5 10
    240607032708_72lq ACTCCAGGGGTGTCTGCTGCCCTTAACTCTAG(+ATATAT)GTACCAAGCATGCACACATACATACATGCAA NGG 10 8 17 8 24 0 100 5 10
    240607032706_err7 CCTGCCCAGCCCCTCCTCCCACAGGAACACAG(+ATATAT)GTACCACCTGCACCCACGACAGCTTCTTTTC NGG 10 8 17 8 24 0 100 5 10
    240607032705_jtkk TGTGAGAAAAGAAGCTGTCGTGGGTGCAGGTG(+ATATAT)GTACCTGTGTTCCTGTGGGAGGAGGGGCTGG NGG 10 8 17 8 24 0 100 5 10
    240607032703_tlbd GTGAGTGTGTGGCTTGCTGTCGTGTCCTCTGG(+ATATAT)GATCCCAAGCCACGCGGGTCTTCCCTCTGTA NGG 10 8 17 8 24 0 100 5 10
    240607032701_i37v CCATCTACAGAGGGAAGACCCGCGTGGCTTGG(+ATATAT)GATCCCAGAGGACACGACAGCAAGCCACACA NGG 10 8 17 8 24 0 100 5 10
    240607032700_qk8o GAAGCCAGTTTCCTAAACACCATGCAGAAGTG(+ATATAT)GTACCCTGGGGCCTGGAAGCTCAAGCTTCCT NGG 10 8 17 8 24 0 100 5 10