Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607032859_km87 ACAGCAGCTGAAGCCATCCCCCATCCCCTCCG(+ATATAT)GTACCCAGTGTAGGTCACAATAACTTAAGAG NGG 10 8 17 8 24 0 100 5 10
    240607032858_va7q TGTGGCACTCAAGCATACTGATCCCCGCTCAG(+ATATAT)GTACCACCATTCCCTATGTAGAGGAGGGAAG NGG 10 8 17 8 24 0 100 5 10
    240607032856_9af8 GGGTGCTTCCCTCCTCTACATAGGGAATGGTG(+ATATAT)GTACCTGAGCGGGGATCAGTATGCTTGAGTG NGG 10 8 17 8 24 0 100 5 10
    240607032855_luq2 ATAGAACACTCAAAAGGGACGTGAATGTGCAG(+ATATAT)GTACCATGGGTCTGGGGTGACCTGCTTCACA NGG 10 8 17 8 24 0 100 5 10
    240607032853_kh8h ACTTCTGTGAAGCAGGTCACCCCAGACCCATG(+ATATAT)GTACCTGCACATTCACGTCCCTTTTGAGTGT NGG 10 8 17 8 24 0 100 5 10
    240607032851_kb2a GCTGCCTGAGGGAGCCTTTGGAGAGCTAACTG(+ATATAT)GTACCCTGATTATAGTCCATTAAGAATCACT NGG 10 8 17 8 24 0 100 5 10
    240607032850_a7cm ACCTAAGTGATTCTTAATGGACTATAATCAGG(+ATATAT)GTACCAGTTAGCTCTCCAAAGGCTCCCTCAG NGG 10 8 17 8 24 0 100 5 10
    240607032848_smnk AGACACAGGTGCAGAGCGGGAAGGCTGACTGG(+ATATAT)GATCCCGCCACCAGCTGTTTTCCCTCTTGTC NGG 10 8 17 8 24 0 100 5 10
    240607032847_s80v GAAATGACAAGAGGGAAAACAGCTGGTGGCGG(+ATATAT)GATCCCAGTCAGCCTTCCCGCTCTGCACCTG NGG 10 8 17 8 24 0 100 5 10
    240607032845_p1yk GGGTGGCCAGGAGCAGCAGAGGTGAAGGGATG(+ATATAT)GATCCACCTGCGGGTCTCTGTGGTTACCGTA NGG 10 8 17 8 24 0 100 5 10