Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607032947_vuoa CATCTCAGGCCAACGGGCTGCCCAAGGCCATG(+ATATAT)GTACCACACACCTGCATCCTGAGGCGGCATT NGG 10 8 17 8 24 0 100 5 10
    240607032946_l0sr AAGCAAATGTAAGGGTATAGGAGCAGCAGCAG(+ATATAT)GATCCTGAGTCACAAGGGCACAATGCCAGCT NGG 10 8 17 8 24 0 100 5 10
    240607032945_6kaa TCTCCAGCTGGCATTGTGCCCTTGTGACTCAG(+ATATAT)GATCCTGCTGCTGCTCCTATACCCTTACATT NGG 10 8 17 8 24 0 100 5 10
    240607032942_deo2 GGCTTCAGTTCTCCCAAAGAGCCAGCCCCTTG(+ATATAT)GATCCAGTGCGCCCAGCCTTTCCCATGTCTA NGG 10 8 17 8 24 0 100 5 10
    240607032941_1bua CGCAGTAGACATGGGAAAGGCTGGGCGCACTG(+ATATAT)GATCCAAGGGGCTGGCTCTTTGGGAGAACTG NGG 10 8 17 8 24 0 100 5 10
    240607032940_3dgb TGGTGAAGTCCTAAGCATGACTTTAAAACCTG(+ATATAT)GATCCAATTTCAAGGGGCTCATGATAAGTGG NGG 10 8 17 8 24 0 100 5 10
    240607032938_bg48 ATATCCCACTTATCATGAGCCCCTTGAAATTG(+ATATAT)GATCCAGGTTTTAAAGTCATGCTTAGGACTT NGG 10 8 17 8 24 0 100 5 10
    240607032937_hwuz AATCTCTGCATGAGTGAAGGGAGGACACATGG(+ATATAT)GTACCGGAATGGGTCTCACAGAAATGGACTT NGG 10 8 17 8 24 0 100 5 10
    240607032935_pnu2 AAAGAAAGTCCATTTCTGTGAGACCCATTCCG(+ATATAT)GTACCCATGTGTCCTCCCTTCACTCATGCAG NGG 10 8 17 8 24 0 100 5 10
    240607032934_x34d AGGCTCCACACACCAGGTATTGCTGATTCCAG(+ATATAT)GTACCACCCTTGACATGTGTGTCTGAGTCCC NGG 10 8 17 8 24 0 100 5 10