Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607033333_bgyx ATCTTGGGAAATCTTGCTGATCTCTCATTTTG(+ATATAT)GTACCCATTTCCGCCTCGGAAGTGTGAGCTC NGG 10 8 17 8 24 0 100 5 10
    240607033332_gys8 TTTCTGTAGGAAATGAAATAACTGATTTTGAG(+ATATAT)GTACCAAATAGTGCAATTGGGTAAGACAGGG NGG 10 8 17 8 24 0 100 5 10
    240607033330_exbh ATAAACCCTGTCTTACCCAATTGCACTATTTG(+ATATAT)GTACCTCAAAATCAGTTATTTCATTTCCTAC NGG 10 8 17 8 24 0 100 5 10
    240607033329_n8b3 GCAGGCATCCTGAGAGGTGCCATGACAACCAG(+ATATAT)GATCCTGTTCACAGCCGATCAGTGTGGAGAG NGG 10 8 17 8 24 0 100 5 10
    240607033327_woes GAAGTCTCTCCACACTGATCGGCTGTGAACAG(+ATATAT)GATCCTGGTTGTCATGGCACCTCTCAGGATG NGG 10 8 17 8 24 0 100 5 10
    240607033326_9qw0 TGAATATAAGGGATCTTACTGTCATTTGTTAG(+ATATAT)GTACCACTGAGTAATTCCATAGTTTCTGTGG NGG 10 8 17 8 24 0 100 5 10
    240607033325_3hoi AATTACCACAGAAACTATGGAATTACTCAGTG(+ATATAT)GTACCTAACAAATGACAGTAAGATCCCTTAT NGG 10 8 17 8 24 0 100 5 10
    240607033322_36hc TGCCTGCAGTTTTAACTTTATAGCTCTCCCAG(+ATATAT)GTACCAGCTTTGGGGCCAGCACACGGCACTC NGG 10 8 17 8 24 0 100 5 10
    240607033321_eb3a AATGAGAGTGCCGTGTGCTGGCCCCAAAGCTG(+ATATAT)GTACCTGGGAGAGCTATAAAGTTAAAACTGC NGG 10 8 17 8 24 0 100 5 10
    240607033320_mcnl CCTCCAGGTGAGACCTGTGATTTCAAGCTTGG(+ATATAT)GTACCACTCTAAGCTTTCTCCGGTCAGCCCA NGG 10 8 17 8 24 0 100 5 10