Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607033451_5egb CTAGCATCCTCTCAGCTGGAGAGGCAGCATTG(+ATATAT)GATCCCCATGCCGCACACGGGGATGGGATGG NGG 10 8 17 8 24 0 100 5 10
    240607033450_ht7e TTCCATACCCTTTCTTCTGTGTTAGCCTGCAG(+ATATAT)GATCCCTTAAGTACCCTTATTCCAATAGGCC NGG 10 8 17 8 24 0 100 5 10
    240607033448_d85y ACCCTGGCCTATTGGAATAAGGGTACTTAAGG(+ATATAT)GATCCTGCAGGCTAACACAGAAGAAAGGGTA NGG 10 8 17 8 24 0 100 5 10
    240607033447_26vx CCGTGGAGCTTTAACACGGAGAATCGAGTTTG(+ATATAT)GATCCCAGAGACATCAGGCAGCCTTGAGACC NGG 10 8 17 8 24 0 100 5 10
    240607033445_1z7b TGCCAGGTCTCAAGGCTGCCTGATGTCTCTGG(+ATATAT)GATCCAAACTCGATTCTCCGTGTTAAAGCTC NGG 10 8 17 8 24 0 100 5 10
    240607033444_70ry CTTGTTCTAATCCTAACCCTAACCCAATTCGG(+ATATAT)GATCCCAGCTGGTGCTCTTAAACACACACAG NGG 10 8 17 8 24 0 100 5 10
    240607033442_82pq AACAACTGTGTGTGTTTAAGAGCACCAGCTGG(+ATATAT)GATCCCGAATTGGGTTAGGGTTAGGATTAGA NGG 10 8 17 8 24 0 100 5 10
    240607033440_kzdl GAAGACAGCCGCTTAGGGAGGGACTACCTTGG(+ATATAT)GATCCAATGAAACCGGCCAGAGAGAGAGAGA NGG 10 8 17 8 24 0 100 5 10
    240607033439_j0h2 CTCTCTCTCTCTCTCTCTGGCCGGTTTCATTG(+ATATAT)GATCCCAAGGTAGTCCCTCCCTAAGCGGCTG NGG 10 8 17 8 24 0 100 5 10
    240607033437_c93z ACCCTACTCTTAGCTGCACCTCTTAGATTCTG(+ATATAT)GTACCTTTCGTTTTATCACCCAGGTGTTGCT NGG 10 8 17 8 24 0 100 5 10