Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607033554_bqaq TAGAGGAGAGAGATTCTTCACTCCCATTAGAG(+ATATAT)GTACCCCTGGTTGGGTGAACAGTGGGGACAG NGG 10 8 17 8 24 0 100 5 10
    240607033553_8l8p CACATCTCCAAAGCCACAGGGACTTGGGTTTG(+ATATAT)GATCCTCCCCATCTGTGTCTACTCCAGCAAT NGG 10 8 17 8 24 0 100 5 10
    240607033552_egj0 AGAGGATTGCTGGAGTAGACACAGATGGGGAG(+ATATAT)GATCCAAACCCAAGTCCCTGTGGCTTTGGAG NGG 10 8 17 8 24 0 100 5 10
    240607033551_w3j3 GATGGTAAGGGGACAGAGGGTGGAAGAGTCAG(+ATATAT)GTACCGCCTGTCCCAAGAGACTAGCCAGGGG NGG 10 8 17 8 24 0 100 5 10
    240607033549_o4dh TAGAGCCCCTGGCTAGTCTCTTGGGACAGGCG(+ATATAT)GTACCTGACTCTTCCACCCTCTGTCCCCTTA NGG 10 8 17 8 24 0 100 5 10
    240607033546_r7hl TTGACCAAGGGTAGGGAAGAGTGAAAGCCCAG(+ATATAT)GATCCGGGCCCGGGATTCCCTGGCTTGGCCA NGG 10 8 17 8 24 0 100 5 10
    240607033544_5861 CAGGGTGGCCAAGCCAGGGAATCCCGGGCCCG(+ATATAT)GATCCTGGGCTTTCACTCTTCCCTACCCTTG NGG 10 8 17 8 24 0 100 5 10
    240607033542_vfu4 GCCCTCCACTCCCTCTGCTAACATTGGGTTGG(+ATATAT)GATCCTCTCTCGGTTGTAACCCATTTCTACC NGG 10 8 17 8 24 0 100 5 10
    240607033541_xyes AATGAGGTAGAAATGGGTTACAACCGAGAGAG(+ATATAT)GATCCCAACCCAATGTTAGCAGAGGGAGTGG NGG 10 8 17 8 24 0 100 5 10
    240607033540_7dtm CAGAGATGTCTGTCAAACATGTATATGCAGCG(+ATATAT)GATCCATCTGCTTGTTTGGTTGGGTTGTTTT NGG 10 8 17 8 24 0 100 5 10