Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607033539_1es9 CAACAAAAACAACCCAACCAAACAAGCAGATG(+ATATAT)GATCCGCTGCATATACATGTTTGACAGACAT NGG 10 8 17 8 24 0 100 5 10
    240607033537_lh7p GTTGTTAGAGCAAAGACACCATTCCATTCCAG(+ATATAT)GTACCAAGTGAAGCATACTTGACAAATAGGC NGG 10 8 17 8 24 0 100 5 10
    240607033536_r70k AGACTGCCTATTTGTCAAGTATGCTTCACTTG(+ATATAT)GTACCTGGAATGGAATGGTGTCTTTGCTCTA NGG 10 8 17 8 24 0 100 5 10
    240607033534_vrz9 TGCAGAGATGGTTTATGAGGCGGGTCCGAAGG(+ATATAT)GTACCCTTCACCTTGCCTGGAGATATACAAA NGG 10 8 17 8 24 0 100 5 10
    240607033532_m261 CAGGTTTTGTATATCTCCAGGCAAGGTGAAGG(+ATATAT)GTACCCTTCGGACCCGCCTCATAAACCATCT NGG 10 8 17 8 24 0 100 5 10
    240607033531_u39l TCCAATCTCGGAAAACAAATCAAAATATTAAG(+ATATAT)GATCCCTGAAATATTAATCCTAATGAGATCT NGG 10 8 17 8 24 0 100 5 10
    240607033529_0yre CAGTGAGATCTCATTAGGATTAATATTTCAGG(+ATATAT)GATCCTTAATATTTTGATTTGTTTTCCGAGA NGG 10 8 17 8 24 0 100 5 10
    240607033528_cwn9 TCTGTGCTCAGGTCACACAGGGGGCTTCAGAG(+ATATAT)GATCCTTATGAAAGATGAGTTAAAAAATGTC NGG 10 8 17 8 24 0 100 5 10
    240607033527_j03e CACTTGACATTTTTTAACTCATCTTTCATAAG(+ATATAT)GATCCTCTGAAGCCCCCTGTGTGACCTGAGC NGG 10 8 17 8 24 0 100 5 10
    240607033525_av49 TTGGTGTTTCCTGACCTGATCTGCAAACTGGG(+ATATAT)GATCCCTGGTCTGTACCTGAATTCTTTCTTT NGG 10 8 17 8 24 0 100 5 10