Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607033658_307r ATACCTCATTAGGTCAGAGGGCCAAGAGTAGG(+ATATAT)GATCCTTGAGGGAATACAGGCTGTTTCAACT NGG 10 8 17 8 24 0 100 5 10
    240607033656_4ej1 TGTCTCTTAAGTGCTAGGATTTAAGGTGTGTG(+ATATAT)GTACCACATCTGGATGGCATTTGGCTTTTTA NGG 10 8 17 8 24 0 100 5 10
    240607033655_vtqb GAAGTTAAAAAGCCAAATGCCATCCAGATGTG(+ATATAT)GTACCACACACCTTAAATCCTAGCACTTAAG NGG 10 8 17 8 24 0 100 5 10
    240607033654_xlc1 AAGGGCCAGAGCTGGGGGGCAAGAAGCTTGTG(+ATATAT)GATCCTTCCCCCCATGCACTGTGAAGGACCC NGG 10 8 17 8 24 0 100 5 10
    240607033652_843b AACGAGGGTCCTTCACAGTGCATGGGGGGAAG(+ATATAT)GATCCACAAGCTTCTTGCCCCCCAGCTCTGG NGG 10 8 17 8 24 0 100 5 10
    240607033651_c1pa CATTCTTTTCACCAGCACCCATCGCCCAAGGG(+ATATAT)GTACCAAGGGGGGCAAGGGGTGTCCAGTCCA NGG 10 8 17 8 24 0 100 5 10
    240607033648_njvz GGGCTTGGACTGGACACCCCTTGCCCCCCTTG(+ATATAT)GTACCCCTTGGGCGATGGGTGCTGGTGAAAA NGG 10 8 17 8 24 0 100 5 10
    240607033646_angc CCATCCTCCCGCAGGAGGAGGGGAACAGGCGG(+ATATAT)GATCCTGGGTCCCTCGTAAGCACTTTGGTTT NGG 10 8 17 8 24 0 100 5 10
    240607033644_e5mz CGGTAAAACCAAAGTGCTTACGAGGGACCCAG(+ATATAT)GATCCCGCCTGTTCCCCTCCTCCTGCGGGAG NGG 10 8 17 8 24 0 100 5 10
    240607033643_0iaj GGTTTCCTTTTCCCCTACTTGACCTTCTATTG(+ATATAT)GTACCCATGAAAAAGCACAAGGTCCTAGCTC NGG 10 8 17 8 24 0 100 5 10