Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607032118_mkox GGACTTGCAGACCCAGCACTCACGGGCACTGG(+ATATAT)GATCCATCCCAGAGAGTGCACCTGAGTAGCT NGG 10 8 17 8 24 0 100 5 10
    240607032116_cf6o GGAAGAGCTACTCAGGTGCACTCTCTGGGATG(+ATATAT)GATCCCAGTGCCCGTGAGTGCTGGGTCTGCA NGG 10 8 17 8 24 0 100 5 10
    240607032114_behf CGGACATGGAATCTTGAAGACCCAAGGCAGGG(+ATATAT)GATCCCAAGGGCTCCATGCAGAACTCACAGG NGG 10 8 17 8 24 0 100 5 10
    240607032112_21xb ATCCTCCTGTGAGTTCTGCATGGAGCCCTTGG(+ATATAT)GATCCCCTGCCTTGGGTCTTCAAGATTCCAT NGG 10 8 17 8 24 0 100 5 10
    240607032110_ctdp TTATTTTTCTAAATAGTGTTCAGAAAACTCTG(+ATATAT)GATCCAAAATGTGGCATTTTTCTGAGAATGA NGG 10 8 17 8 24 0 100 5 10
    240607032109_228x AATCTTCATTCTCAGAAAAATGCCACATTTTG(+ATATAT)GATCCAGAGTTTTCTGAACACTATTTAGAAA NGG 10 8 17 8 24 0 100 5 10
    240607032108_xrn0 ACTAAGCTGATCTGGCTATCCCAATGCATGTG(+ATATAT)GTACCTGATAGATGGATATGGTAGAGATTAT NGG 10 8 17 8 24 0 100 5 10
    240607032107_92ro GGGAAATAATCTCTACCATATCCATCTATCAG(+ATATAT)GTACCACATGCATTGGGATAGCCAGATCAGC NGG 10 8 17 8 24 0 100 5 10
    240607032105_2ont TTATCATCTCTGACCTTCTGAAGCCAGGGGTG(+ATATAT)GTACCCATCCCTAGCTCAGTCTAATTAAATC NGG 10 8 17 8 24 0 100 5 10
    240607032103_jmnm GGAAAGATTTAATTAGACTGAGCTAGGGATGG(+ATATAT)GTACCACCCCTGGCTTCAGAAGGTCAGAGAT NGG 10 8 17 8 24 0 100 5 10