Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607032022_wwn4 CAAAGCATGCTTACTTGATGCTTTATAAGTGG(+ATATAT)GATCCAGGATGGGGAATGCAAATTAGTTATA NGG 10 8 17 8 24 0 100 5 10
    240607032020_7vpl ATATTTATAACTAATTTGCATTCCCCATCCTG(+ATATAT)GATCCCACTTATAAAGCATCAAGTAAGCATG NGG 10 8 17 8 24 0 100 5 10
    240607032018_g59j GTATGTCTAGTTCCTCTCACTTGACCCTAGGG(+ATATAT)GATCCTAATCCTTGCCATTTCATAGATATTT NGG 10 8 17 8 24 0 100 5 10
    240607032017_z08u CTTTGAAATATCTATGAAATGGCAAGGATTAG(+ATATAT)GATCCCCTAGGGTCAAGTGAGAGGAACTAGA NGG 10 8 17 8 24 0 100 5 10
    240607032015_j1e7 AGCAATTGCTTCCGTAGTGTCTGGACATCCGG(+ATATAT)GTACCCGGGCATCAAATCCAGGAGCCAAAGG NGG 10 8 17 8 24 0 100 5 10
    240607032012_2ecc AGGCACCTTTGGCTCCTGGATTTGATGCCCGG(+ATATAT)GTACCCGGATGTCCAGACACTACGGAAGCAA NGG 10 8 17 8 24 0 100 5 10
    240607032010_9jn6 GAAATGTTTAAATTAACTGTGTTGTACAAATG(+ATATAT)GTACCCAACACAAAACCTTTTTTAAATTAGT NGG 10 8 17 8 24 0 100 5 10
    240607032009_cbsx GTCTTACTAATTTAAAAAAGGTTTTGTGTTGG(+ATATAT)GTACCATTTGTACAACACAGTTAATTTAAAC NGG 10 8 17 8 24 0 100 5 10
    240607032007_luls TGGTGGGCTCAGGTTTGCTTGGTCTTGTGTGG(+ATATAT)GTACCCATTGCCACTTTGAGTTTATGGGTTT NGG 10 8 17 8 24 0 100 5 10
    240607032005_j641 TAGCTAAACCCATAAACTCAAAGTGGCAATGG(+ATATAT)GTACCCACACAAGACCAAGCAAACCTGAGCC NGG 10 8 17 8 24 0 100 5 10