Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607031949_orgt TCTCAAGGTCTTGGTCAGGACCTCACTACCAG(+ATATAT)GTACCCGCACCCCCAACCTTTCAATAGCAAG NGG 10 8 17 8 24 0 100 5 10
    240607031947_bb5y GGGTTCTTGCTATTGAAAGGTTGGGGGTGCGG(+ATATAT)GTACCTGGTAGTGAGGTCCTGACCAAGACCT NGG 10 8 17 8 24 0 100 5 10
    240607031946_8wt0 TCCTATTTCTCCTTGAATATTAACTTGGAAAG(+ATATAT)GTACCCAGTAAGCCAAGCACTGCCCAGACAG NGG 10 8 17 8 24 0 100 5 10
    240607031945_ws8y CTGGTCTGTCTGGGCAGTGCTTGGCTTACTGG(+ATATAT)GTACCTTTCCAAGTTAATATTCAAGGAGAAA NGG 10 8 17 8 24 0 100 5 10
    240607031943_kc8r TTCATTGACCAACCAGTCTTAAGAATGAAGTG(+ATATAT)GTACCCAGGCTCATTGAGAGATCCTGTCTCA NGG 10 8 17 8 24 0 100 5 10
    240607031941_gmnr TTTTTTGAGACAGGATCTCTCAATGAGCCTGG(+ATATAT)GTACCACTTCATTCTTAAGACTGGTTGGTCA NGG 10 8 17 8 24 0 100 5 10
    240607031940_pwwg ATATGGCTATAAATATAAATTTTGTATTACTG(+ATATAT)GTACCAAAAACACTTTTTTTTTTTTTTCCAG NGG 10 8 17 8 24 0 100 5 10
    240607031939_qf84 CCCTGCTGGAAAAAAAAAAAAAAGTGTTTTTG(+ATATAT)GTACCAGTAATACAAAATTTATATTTATAGC NGG 10 8 17 8 24 0 100 5 10
    240607031937_pnz4 CTAGGTGTTCAGGGACCTCCAAAAGCTCCAAG(+ATATAT)GATCCACCTCTTGCTACATCTTCAGTAGGAA NGG 10 8 17 8 24 0 100 5 10
    240607031936_y91g CTCTATTCCTACTGAAGATGTAGCAAGAGGTG(+ATATAT)GATCCTTGGAGCTTTTGGAGGTCCCTGAACA NGG 10 8 17 8 24 0 100 5 10