Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607031904_ifdd CTACGTTCATCCAGGCAGCAACAGAAGCTACG(+ATATAT)GATCCTTAACATATCAATGAGCTCTGAACTT NGG 10 8 17 8 24 0 100 5 10
    240607031903_uq7o TATTTAAGTTCAGAGCTCATTGATATGTTAAG(+ATATAT)GATCCGTAGCTTCTGTTGCTGCCTGGATGAA NGG 10 8 17 8 24 0 100 5 10
    240607031902_g8gn GGTGGAGTGGATTGATTGGAGGCTGAGAAAAG(+ATATAT)GTACCAAAGCACAGAGGTTGAAACAATAAAT NGG 10 8 17 8 24 0 100 5 10
    240607031900_6d1m CTAGAATTTATTGTTTCAACCTCTGTGCTTTG(+ATATAT)GTACCTTTTCTCAGCCTCCAATCAATCCACT NGG 10 8 17 8 24 0 100 5 10
    240607031859_z92z AAAAACCTTGATAATAGGCTAGGTTGTTGGAG(+ATATAT)GATCCCATGGGGCCCCTTCAGCCAACAACTA NGG 10 8 17 8 24 0 100 5 10
    240607031857_pyki TAATCTAGTTGTTGGCTGAAGGGGCCCCATGG(+ATATAT)GATCCTCCAACAACCTAGCCTATTATCAAGG NGG 10 8 17 8 24 0 100 5 10
    240607031855_2v7n CAGTCAACCTCAGGAGGTCGGATGTTGAGGGG(+ATATAT)GATCCTCCATAATGTACCAGAGACCTGGGAA NGG 10 8 17 8 24 0 100 5 10
    240607031853_6ycn CTCACTTCCCAGGTCTCTGGTACATTATGGAG(+ATATAT)GATCCCCCTCAACATCCGACCTCCTGAGGTT NGG 10 8 17 8 24 0 100 5 10
    240607031852_xvo4 AAATCAGTAATGTTATCTAGCTAAAAGAATAG(+ATATAT)GTACCCTGCATTATATTATGTAAGATTAAAA NGG 10 8 17 8 24 0 100 5 10
    240607031851_5qvh TTTGTTTTTAATCTTACATAATATAATGCAGG(+ATATAT)GTACCTATTCTTTTAGCTAGATAACATTACT NGG 10 8 17 8 24 0 100 5 10