Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607031834_7ou1 CCCATCCACCTGGCATTTATTTGGCTTCTAGG(+ATATAT)GATCCAAACTCCGGCTCTCTTCACGCATGGC NGG 10 8 17 8 24 0 100 5 10
    240607031833_6l3z TACTTGCCATGCGTGAAGAGAGCCGGAGTTTG(+ATATAT)GATCCCTAGAAGCCAAATAAATGCCAGGTGG NGG 10 8 17 8 24 0 100 5 10
    240607031831_9h06 GTGTGGAGCTTGGCAGCCTCTACCTGGAGTGG(+ATATAT)GTACCATCTGCCTGACGTTGCAACTGATAGC NGG 10 8 17 8 24 0 100 5 10
    240607031830_cdwa CCGTGGCTATCAGTTGCAACGTCAGGCAGATG(+ATATAT)GTACCCACTCCAGGTAGAGGCTGCCAAGCTC NGG 10 8 17 8 24 0 100 5 10
    240607031829_co60 GTGGTTAGGGTGTATAAAACCTGAACTTGCAG(+ATATAT)GATCCGGTGAAGCCTTTATTTAGCATGTGTG NGG 10 8 17 8 24 0 100 5 10
    240607031827_o6f5 GTAGCCACACATGCTAAATAAAGGCTTCACCG(+ATATAT)GATCCTGCAAGTTCAGGTTTTATACACCCTA NGG 10 8 17 8 24 0 100 5 10
    240607031826_yfs6 CAACCCACAAGCAGGTGTGTGAACCAGAATGG(+ATATAT)GATCCGCTGATGGCTGGCATGCCTTGGGAGA NGG 10 8 17 8 24 0 100 5 10
    240607031825_5vzu TAGCCTCTCCCAAGGCATGCCAGCCATCAGCG(+ATATAT)GATCCCATTCTGGTTCACACACCTGCTTGTG NGG 10 8 17 8 24 0 100 5 10
    240607031822_vf1o GTCATAACCCCCACCCCACCCCACCCCCGGTG(+ATATAT)GTACCTTAAAGAGATTAAATGCAAACATTCC NGG 10 8 17 8 24 0 100 5 10
    240607031821_sjqv AGCAAGGAATGTTTGCATTTAATCTCTTTAAG(+ATATAT)GTACCACCGGGGGTGGGGTGGGGTGGGGGTT NGG 10 8 17 8 24 0 100 5 10