Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607031733_ifyi CTTCTGATTGATGGTACCATCCCCCAAGAAGG(+ATATAT)GATCCTCTCAGGAAGAAATGCAGGCTTTCTA NGG 10 8 17 8 24 0 100 5 10
    240607031732_4o6a GAGTTTAGAAAGCCTGCATTTCTTCCTGAGAG(+ATATAT)GATCCCTTCTTGGGGGATGGTACCATCAATC NGG 10 8 17 8 24 0 100 5 10
    240607031731_6t4b CCTCTGTAGACCAGGCTGGCCATAAACTCAGG(+ATATAT)GATCCACCTGCCTCTACTTTCGGAGTGCTGA NGG 10 8 17 8 24 0 100 5 10
    240607031729_dy2w TAATCTCAGCACTCCGAAAGTAGAGGCAGGTG(+ATATAT)GATCCCTGAGTTTATGGCCAGCCTGGTCTAC NGG 10 8 17 8 24 0 100 5 10
    240607031727_aebz GGTGTCAGCGTTTGGATGCTGATTATGGGGTG(+ATATAT)GATCCCTGGATATGGCAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10
    240607031725_xfej GGATGGACCATGTAGAGACTGCCATATCCAGG(+ATATAT)GATCCACCCCATAATCAGCATCCAAACGCTG NGG 10 8 17 8 24 0 100 5 10
    240607031724_de90 TTCTGGATATATGCCCAGGAGAGGTATTGCTG(+ATATAT)GATCCTCCGGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240607031721_fg3y TCCTCAGAAAATTGGACATAGTACTACCGGAG(+ATATAT)GATCCAGCAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10
    240607031720_xe40 GTGTCCTGGATTACCTGGATGTTTTGAGTTAG(+ATATAT)GATCCTTTTGCATTTTGTATTTTCTTTGACT NGG 10 8 17 8 24 0 100 5 10
    240607031719_f3te ACAACAGTCAAAGAAAATACAAAATGCAAAAG(+ATATAT)GATCCTAACTCAAAACATCCAGGTAATCCAG NGG 10 8 17 8 24 0 100 5 10