Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607031645_4uts CACCAAGCCTGATATCTGTGTTTGAACCCTAG(+ATATAT)GATCCATATGGTAGGAGAGAACTAACCCACA NGG 10 8 17 8 24 0 100 5 10
    240607031644_7t0q CAACTTGTGGGTTAGTTCTCTCCTACCATATG(+ATATAT)GATCCTAGGGTTCAAACACAGATATCAGGCT NGG 10 8 17 8 24 0 100 5 10
    240607031642_6j5s TGGGTTTGGACTCCTTGTCTACATCTGTCCCG(+ATATAT)GATCCTCAACGCTCTCCAAAGGACAGCCTAC NGG 10 8 17 8 24 0 100 5 10
    240607031641_lvnb CGGTGGTAGGCTGTCCTTTGGAGAGCGTTGAG(+ATATAT)GATCCGGGACAGATGTAGACAAGGAGTCCAA NGG 10 8 17 8 24 0 100 5 10
    240607031639_hcl3 TATATTTAAAAAACAAAAACAGTCTTGCCATG(+ATATAT)GATCCAGTGTTAACACTGTCATTTTGTAACA NGG 10 8 17 8 24 0 100 5 10
    240607031638_9mr9 AAGGCTGTTACAAAATGACAGTGTTAACACTG(+ATATAT)GATCCATGGCAAGACTGTTTTTGTTTTTTAA NGG 10 8 17 8 24 0 100 5 10
    240607031636_wxkl AGACCCCACACTTTTTGTAAGACATCAATCAG(+ATATAT)GATCCCCATATTAATTATAAGCATCACTGAA NGG 10 8 17 8 24 0 100 5 10
    240607031635_51tn CAACATTCAGTGATGCTTATAATTAATATGGG(+ATATAT)GATCCTGATTGATGTCTTACAAAAAGTGTGG NGG 10 8 17 8 24 0 100 5 10
    240607031633_7m2d TGACACTGAAGGAATAGGCAGAGGTTTTGAAG(+ATATAT)GTACCTCATAGCTACCACAATGATAATAACA NGG 10 8 17 8 24 0 100 5 10
    240607031632_zhwn GTGGTTGTTATTATCATTGTGGTAGCTATGAG(+ATATAT)GTACCTTCAAAACCTCTGCCTATTCCTTCAG NGG 10 8 17 8 24 0 100 5 10