Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607031615_9r5q CCAAAAAATACAGCAAAGACAGGAACGTCCAG(+ATATAT)GATCCGGGTCTGTTAATAAAGCCACCTCGAG NGG 10 8 17 8 24 0 100 5 10
    240607031613_eh0l CTAGTCTCGAGGTGGCTTTATTAACAGACCCG(+ATATAT)GATCCTGGACGTTCCTGTCTTTGCTGTATTT NGG 10 8 17 8 24 0 100 5 10
    240607031611_98zg ATCAGGCTGGGGAGGTTAGTGCACAATCAGTG(+ATATAT)GATCCATGCCCCGCCTCCAAGCTCTGCAAAC NGG 10 8 17 8 24 0 100 5 10
    240607031610_ahdn GTGGGGTTTGCAGAGCTTGGAGGCGGGGCATG(+ATATAT)GATCCACTGATTGTGCACTAACCTCCCCAGC NGG 10 8 17 8 24 0 100 5 10
    240607031609_0z1x CAGAAGTCAGAGTCATCAGTCATTTGGCTAAG(+ATATAT)GTACCGCTGAGACACAATATCTCTGTAACTT NGG 10 8 17 8 24 0 100 5 10
    240607031608_k4vh AAAGAAAGTTACAGAGATATTGTGTCTCAGCG(+ATATAT)GTACCTTAGCCAAATGACTGATGACTCTGAC NGG 10 8 17 8 24 0 100 5 10
    240607031606_dumo GGAAAAAACTGAAACACTAAGTGTAGGAAGAG(+ATATAT)GATCCTAGAAAACTAAACGTATTGACTTTGA NGG 10 8 17 8 24 0 100 5 10
    240607031605_4vdo TTATTTCAAAGTCAATACGTTTAGTTTTCTAG(+ATATAT)GATCCTCTTCCTACACTTAGTGTTTCAGTTT NGG 10 8 17 8 24 0 100 5 10
    240607031604_94ib CTTCACACAACATCTGCATTCACATATTTTAG(+ATATAT)GATCCTTTGTCTCTCTTCCTGTACACTTTTT NGG 10 8 17 8 24 0 100 5 10
    240607031603_pwbr AGATGAAAAAGTGTACAGGAAGAGAGACAAAG(+ATATAT)GATCCTAAAATATGTGAATGCAGATGTTGTG NGG 10 8 17 8 24 0 100 5 10