Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607031701_wqz7 TGGGTTTGTTTTCCCTGAACCCCCTCCCCCAG(+ATATAT)GATCCAGACTTCCCACTTATATAGCTTATCA NGG 10 8 17 8 24 0 100 5 10
    240607031700_fntb ATAGTTGATAAGCTATATAAGTGGGAAGTCTG(+ATATAT)GATCCTGGGGGAGGGGGTTCAGGGAAAACAA NGG 10 8 17 8 24 0 100 5 10
    240607031658_wg3y CATGTCTGTCAGGCATTTATATAAATTTTGGG(+ATATAT)GATCCAAACTCTGGATTTTATTGTTATTTTT NGG 10 8 17 8 24 0 100 5 10
    240607031657_lib6 AGCACAAAAATAACAATAAAATCCAGAGTTTG(+ATATAT)GATCCCCAAAATTTATATAAATGCCTGACAG NGG 10 8 17 8 24 0 100 5 10
    240607031656_7zye GACTTTATGTGGGAGCTGGGGATTTGAACTAG(+ATATAT)GATCCTTGTATAGCAAGCATCCATATTCTGA NGG 10 8 17 8 24 0 100 5 10
    240607031654_l1fy ATGGTTCAGAATATGGATGCTTGCTATACAAG(+ATATAT)GATCCTAGTTCAAATCCCCAGCTCCCACATA NGG 10 8 17 8 24 0 100 5 10
    240607031653_89vq TTGGATTAAAAGCCTGTTTATCTCCGTATTTG(+ATATAT)GATCCCTGGATTCTATAGCCTTGCCAAAGAC NGG 10 8 17 8 24 0 100 5 10
    240607031651_h3hd GTTGAGTCTTTGGCAAGGCTATAGAATCCAGG(+ATATAT)GATCCAAATACGGAGATAAACAGGCTTTTAA NGG 10 8 17 8 24 0 100 5 10
    240607031649_i9mo TCAAAGACCTCAAGAACTGCCTATGGACCCAG(+ATATAT)GTACCTTCCCAATGCATGAGCTACAAACCCA NGG 10 8 17 8 24 0 100 5 10
    240607031648_79co GAAGGTGGGTTTGTAGCTCATGCATTGGGAAG(+ATATAT)GTACCTGGGTCCATAGGCAGTTCTTGAGGTC NGG 10 8 17 8 24 0 100 5 10