Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607031631_yi24 CACACATCTGTGCACTGACTTTGCCATTGAGG(+ATATAT)GATCCTCTGGGTTGGGTCTTTAAGACGAAGG NGG 10 8 17 8 24 0 100 5 10
    240607031628_48od GTTTCCCTTCGTCTTAAAGACCCAACCCAGAG(+ATATAT)GATCCCTCAATGGCAAAGTCAGTGCACAGAT NGG 10 8 17 8 24 0 100 5 10
    240607031627_efhc GCCCTTCCCACCTTTGCCTGTCTACAGTAGTG(+ATATAT)GATCCATTTCCTCCCGAGGTTGCCCATCAAT NGG 10 8 17 8 24 0 100 5 10
    240607031626_rq25 ATCCAATTGATGGGCAACCTCGGGAGGAAATG(+ATATAT)GATCCACTACTGTAGACAGGCAAAGGTGGGA NGG 10 8 17 8 24 0 100 5 10
    240607031625_60p3 ATACTCCCAGAATGAGCAGAGGGTCCAAAGTG(+ATATAT)GATCCCTGAGCCATTTTGAATAATTTTATCA NGG 10 8 17 8 24 0 100 5 10
    240607031623_sja5 AGTGGTGATAAAATTATTCAAAATGGCTCAGG(+ATATAT)GATCCACTTTGGACCCTCTGCTCATTCTGGG NGG 10 8 17 8 24 0 100 5 10
    240607031621_1mfw TAGCACAGGGTCCAGGCAACCTCAGCCTATGG(+ATATAT)GATCCTTAGGCACTGTGTCACAGGAACGTCC NGG 10 8 17 8 24 0 100 5 10
    240607031619_x50a AGCTAGGACGTTCCTGTGACACAGTGCCTAAG(+ATATAT)GATCCCATAGGCTGAGGTTGCCTGGACCCTG NGG 10 8 17 8 24 0 100 5 10
    240607031618_69vd GTTTCAAGATTTTCTAACACAAATTTACATTG(+ATATAT)GTACCAAAATGGGCAAGAAAATGAAGGCACA NGG 10 8 17 8 24 0 100 5 10
    240607031617_rth8 GAGCCTGTGCCTTCATTTTCTTGCCCATTTTG(+ATATAT)GTACCAATGTAAATTTGTGTTAGAAAATCTT NGG 10 8 17 8 24 0 100 5 10