Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607031802_4er8 AAAATAAGTACAACATCTGATGTCCAAACAGG(+ATATAT)GATCCTTCTGGGTGTGGCTTTACCCATGCAA NGG 10 8 17 8 24 0 100 5 10
    240607031801_38lo TGCAGTTGCATGGGTAAAGCCACACCCAGAAG(+ATATAT)GATCCCTGTTTGGACATCAGATGTTGTACTT NGG 10 8 17 8 24 0 100 5 10
    240607031800_w0bq AAGGGTAGCAACAGCTTGTCTTCTGATTGATG(+ATATAT)GTACCATCCCCCAAGAAGGGATCCTCTCAGG NGG 10 8 17 8 24 0 100 5 10
    240607031759_a94f TTCTTCCTGAGAGGATCCCTTCTTGGGGGATG(+ATATAT)GTACCATCAATCAGAAGACAAGCTGTTGCTA NGG 10 8 17 8 24 0 100 5 10
    240607031757_ckcv GGAGCTATGGAAGCATTGGTCTCTACACAGCG(+ATATAT)GTACCCACAGGACCCACACAATGAGGGTTAG NGG 10 8 17 8 24 0 100 5 10
    240607031756_6ib6 AAATGCTAACCCTCATTGTGTGGGTCCTGTGG(+ATATAT)GTACCGCTGTGTAGAGACCAATGCTTCCATA NGG 10 8 17 8 24 0 100 5 10
    240607031755_i7z0 TAGATGATCAACTTGATCCTGATTCAGCTTTG(+ATATAT)GTACCTGGTATCTGTCCAGAAATTTGTCCAT NGG 10 8 17 8 24 0 100 5 10
    240607031753_aqbu ACGAAATGGACAAATTTCTGGACAGATACCAG(+ATATAT)GTACCAAAGCTGAATCAGGATCAAGTTGATC NGG 10 8 17 8 24 0 100 5 10
    240607031751_xs62 CTCTGCAGGCAAGCTCTCTTCTTGCAGGGCAG(+ATATAT)GTACCCAGATATCTGGTGTTCGAACCAGACT NGG 10 8 17 8 24 0 100 5 10
    240607031750_rsh0 CCAGGAGTCTGGTTCGAACACCAGATATCTGG(+ATATAT)GTACCTGCCCTGCAAGAAGAGAGCTTGCCTG NGG 10 8 17 8 24 0 100 5 10