Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607031518_eymd ATAGAAAGCAGGGGCTGGGTGTAACCCAGTTG(+ATATAT)GTACCTACAGTTGCCTGGCATGGATGAAGCC NGG 10 8 17 8 24 0 100 5 10
    240607031517_1y7g TTCAGGGCTTCATCCATGCCAGGCAACTGTAG(+ATATAT)GTACCAACTGGGTTACACCCAGCCCCTGCTT NGG 10 8 17 8 24 0 100 5 10
    240607031515_ln37 TTCACTTGAGATGCCAAGTATTGTACTTTCGG(+ATATAT)GATCCTTTAAAAGGCCATTTTGCTCATTTCA NGG 10 8 17 8 24 0 100 5 10
    240607031514_k6wg TGGGTTGAAATGAGCAAAATGGCCTTTTAAAG(+ATATAT)GATCCCGAAAGTACAATACTTGGCATCTCAA NGG 10 8 17 8 24 0 100 5 10
    240607031512_2z21 GCTGGTCTAAGAGAAGTGCGGAAGCAAAATGG(+ATATAT)GATCCTTAAGCCAGCTGTGGTGGCTCAGGTT NGG 10 8 17 8 24 0 100 5 10
    240607031511_geav TAATCAACCTGAGCCACCACAGCTGGCTTAAG(+ATATAT)GATCCCATTTTGCTTCCGCACTTCTCTTAGA NGG 10 8 17 8 24 0 100 5 10
    240607031510_924d CCCTAAATATAGGATGGGAATCCTTGGGATAG(+ATATAT)GATCCTGAGTTAAGTAAGGGGGTGAAAGCAA NGG 10 8 17 8 24 0 100 5 10
    240607031509_px99 TCAGCTTGCTTTCACCCCCTTACTTAACTCAG(+ATATAT)GATCCTATCCCAAGGATTCCCATCCTATATT NGG 10 8 17 8 24 0 100 5 10
    240607031508_96f6 GATTCACTACAGTGTGAGCAACTCGGCTCTTG(+ATATAT)GATCCAAAGCAGCACTTCATATATAAAAGGG NGG 10 8 17 8 24 0 100 5 10
    240607031506_2u9k GTTTGCCCTTTTATATATGAAGTGCTGCTTTG(+ATATAT)GATCCAAGAGCCGAGTTGCTCACACTGTAGT NGG 10 8 17 8 24 0 100 5 10