Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607031425_vzat TATGAGTGGATTTAAGTGAAATTTTTCATATG(+ATATAT)GATCCACATTAACTTAGAATACATTATAATA NGG 10 8 17 8 24 0 100 5 10
    240607031424_h99q TTGTTTATTATAATGTATTCTAAGTTAATGTG(+ATATAT)GATCCATATGAAAAATTTCACTTAAATCCAC NGG 10 8 17 8 24 0 100 5 10
    240607031423_d3wo CTGTGTGACAACCAGCATGTCTAACCTTTGAG(+ATATAT)GATCCTGACCCTGGTTAGGCTTTGCTTTAGA NGG 10 8 17 8 24 0 100 5 10
    240607031422_t6al GTGTATCTAAAGCAAAGCCTAACCAGGGTCAG(+ATATAT)GATCCTCAAAGGTTAGACATGCTGGTTGTCA NGG 10 8 17 8 24 0 100 5 10
    240607031421_7zc8 CTATTGATAAATAGCTTCAGCTACACAGGTTG(+ATATAT)GTACCCACCAGCCAGCAGGATTTTTTGGTTA NGG 10 8 17 8 24 0 100 5 10
    240607031419_h03j AGCAATAACCAAAAAATCCTGCTGGCTGGTGG(+ATATAT)GTACCAACCTGTGTAGCTGAAGCTATTTATC NGG 10 8 17 8 24 0 100 5 10
    240607031418_gkge GGCAAAGAACCCTGACAGTGGCAAACAAGCAG(+ATATAT)GATCCCCCAGGCGCTGGCCCTAGAGCCTAAT NGG 10 8 17 8 24 0 100 5 10
    240607031415_jits TATCAATTAGGCTCTAGGGCCAGCGCCTGGGG(+ATATAT)GATCCTGCTTGTTTGCCACTGTCAGGGTTCT NGG 10 8 17 8 24 0 100 5 10
    240607031414_t326 CACTGACAAGTGATATTAGCCATAAAGTGCAG(+ATATAT)GTACCATGCCACACTCCACAGACCAAGGAGG NGG 10 8 17 8 24 0 100 5 10
    240607031413_in70 TTTCACCTCCTTGGTCTGTGGAGTGTGGCATG(+ATATAT)GTACCTGCACTTTATGGCTAATATCACTTGT NGG 10 8 17 8 24 0 100 5 10