Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607031356_1j9p GACACATAGCTCAGGAACACTGAATTCATTCG(+ATATAT)GTACCAGGAAATGTGAATAATTTATATGTTT NGG 10 8 17 8 24 0 100 5 10
    240607031354_6xrg TCCTTAAACATATAAATTATTCACATTTCCTG(+ATATAT)GTACCGAATGAATTCAGTGTTCCTGAGCTAT NGG 10 8 17 8 24 0 100 5 10
    240607031353_z84g TAATGGGCTGAAAACAGTAACAAGGCAGGCTG(+ATATAT)GTACCCAAAGGTGAAAGTATTTCCCTTCAAA NGG 10 8 17 8 24 0 100 5 10
    240607031352_akdg ATATTTTTGAAGGGAAATACTTTCACCTTTGG(+ATATAT)GTACCAGCCTGCCTTGTTACTGTTTTCAGCC NGG 10 8 17 8 24 0 100 5 10
    240607031349_67yn TTTCCCAGTCCGGCATCCTTTTCATAGCCCTG(+ATATAT)GATCCGTTGGAAGGTTTACCTAAGTTAACTG NGG 10 8 17 8 24 0 100 5 10
    240607031347_pa3w TTTTGCAGTTAACTTAGGTAAACCTTCCAACG(+ATATAT)GATCCAGGGCTATGAAAAGGATGCCGGACTG NGG 10 8 17 8 24 0 100 5 10
    240607031346_6lc6 CATAACAGTTCATATTGACCTTTGATTTAATG(+ATATAT)GATCCTACATAAAGCTTTGTGGAATTTCTTA NGG 10 8 17 8 24 0 100 5 10
    240607031345_bbil AATCTTAAGAAATTCCACAAAGCTTTATGTAG(+ATATAT)GATCCATTAAATCAAAGGTCAATATGAACTG NGG 10 8 17 8 24 0 100 5 10
    240607031343_4hkn CTGTGAAGCCCCTGTTATATACTACCTGGTGG(+ATATAT)GTACCTTGCCAACTTTTCCTGTGAAACCAGA NGG 10 8 17 8 24 0 100 5 10
    240607031342_hgak GTTGATCTGGTTTCACAGGAAAAGTTGGCAAG(+ATATAT)GTACCCACCAGGTAGTATATAACAGGGGCTT NGG 10 8 17 8 24 0 100 5 10