Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607031326_d1p8 CAGCCCAACTGGACCAGAGCTCAGGGGTCCTG(+ATATAT)GTACCTTTCTTCCCATCATAGACAACATTAT NGG 10 8 17 8 24 0 100 5 10
    240607031324_mhuk TTTTAATAATGTTGTCTATGATGGGAAGAAAG(+ATATAT)GTACCAGGACCCCTGAGCTCTGGTCCAGTTG NGG 10 8 17 8 24 0 100 5 10
    240607031323_c612 GGCATGAAGGAGGAAAGAGCATCTTGGAGCTG(+ATATAT)GTACCCAACCAAGGCTTGCTTGTTCCCCAAA NGG 10 8 17 8 24 0 100 5 10
    240607031322_c2fo GCTTCTTTGGGGAACAAGCAAGCCTTGGTTGG(+ATATAT)GTACCAGCTCCAAGATGCTCTTTCCTCCTTC NGG 10 8 17 8 24 0 100 5 10
    240607031320_8q9u GAGGCTTCAGCGAGCATCGATGTCTGCCTCAG(+ATATAT)GATCCTTACATCCCTGCATAGCATCAGTGTG NGG 10 8 17 8 24 0 100 5 10
    240607031319_mb9l GTGTACACACTGATGCTATGCAGGGATGTAAG(+ATATAT)GATCCTGAGGCAGACATCGATGCTCGCTGAA NGG 10 8 17 8 24 0 100 5 10
    240607031317_yx2i GGTTACCTACCCACCACCCCTCCAGGCACCAG(+ATATAT)GATCCCCTATTTAGCTTCCTGTAACGCCCCC NGG 10 8 17 8 24 0 100 5 10
    240607031315_j2uf GCTGTGGGGGCGTTACAGGAAGCTAAATAGGG(+ATATAT)GATCCTGGTGCCTGGAGGGGTGGTGGGTAGG NGG 10 8 17 8 24 0 100 5 10
    240607031314_j1bw GCTTTGGTATAGTGAATGTCTCCCTGTGCAGG(+ATATAT)GTACCACGCCATGTGCCATTTAGGCACAAGC NGG 10 8 17 8 24 0 100 5 10
    240607031313_cugl GTCTGGCTTGTGCCTAAATGGCACATGGCGTG(+ATATAT)GTACCCTGCACAGGGAGACATTCACTATACC NGG 10 8 17 8 24 0 100 5 10